Figure_1.tif (3.36 MB)

CD3 and CD68 positive cells accumulate in the neo-terminal ileum of Crohn’s disease patients.

figure

posted on 2013-02-19, 16:57 authored by Francesca Zorzi, Ivan Monteleone, Massimiliano Sarra, Emma Calabrese, Irene Marafini, Micaela Cretella, Silvia Sedda, Livia Biancone, Francesco Pallone, Giovanni Monteleone

Representative immunofluorescence pictures of ileal sections of 1 CD patient with no evidence of endoscopic recurrence (i0), 1 CD patient with severe endoscopic recurrence (i4), 1 CD patient with established (late) lesion and 1normal control and stained with CD3+/DAPI (A) and CD68+/DAPI (B). Original magnification 100x. Insets in the left images show CD3 positive cells (A) and CD68 positive cells (B) at higher magnification (200x). C–D. Quantification of CD3+ and CD68+ cells in intestinal mucosa of 5 CD patients with no endoscopic recurrence (i0–i1), 5 CD patients with endoscopic recurrence (i2–i4), 5 CD patients with established lesions and 5 normal controls. Data are presented as mean values of positive cells per high power field ± SD of 5 independent experiments in which 5 sections per group were analyzed.