CCR2 does not contribute to liver extravasation of CD11b⁺Ly6C⁺ monocytic cells during T. brucei infection.

WT and CCR2 KO CD11b⁺Ly6C⁺ monocytic cells were isolated from bone marrow of mice on day 6 of infection, labeled with PKH26 and CellVue kits, respectively, and injected at a 1-1 ratio in the blood of recipient WT mice on day 6 of T. brucei infection. Left panel shows percentages of gated, labeled WT and CCR2 KO cells before injection. 24 hours after transfer liver non-parenchymal were harvested from recipient mice and analysed for the presence of labeled cells. Percentages of gated, labeled WT and CCR2 KO cells are shown (right panel). FACS profiles are representative of one of six animals tested in two independent experiments.