Breadth of detection of the cpn60-targeted PCR assays for ‘Ca.Phytoplasma’ spp.

A. Samples amplified using primer set H279p/H280p. B. Samples amplified using primer set D0317/D0318. C. Samples amplified using an optimized cocktail consisting of a 1∶7 molar ratio of primer sets H279p/H280p:D0317/D0318. For all panels: Lane 1, Apple proliferation (‘Ca.P. mali’); lane 2, Peach yellow leaf roll (‘Ca.P. pyri’); lane 3, European stone fruit yellows (‘Ca.P. prunorum’); lane 4, Bois noir – isolate Pyrenées Orientalis (‘Ca.P. solani’); lane 5, AY strain OY-M (‘Ca.P. asteris’); lane 6, AY strain COL (‘Ca.P. asteris’); lane 7, AY strain CVB (‘Ca.P. asteris’); lane 8, AY strain AY-WB (‘Ca.P. asteris’); lane 9, Brazilian huanglongbing phytoplasma (‘Ca.P. phoenicium’); lane 10, Flavescence dorée (‘Ca.P. ulmi’); lane 11, Bois noir – isolate VL-06-1-20, Lebanon (‘Ca.P. solani’); lane 12, Rubus stunt (‘Ca.P. ulmi’); lane 13, Ash yellows (‘Ca.P. fraxini’); lane 14, no template control.