posted on 2013-04-05, 01:45authored byAnna V. Miller, Mark A. Hicks, Wataru Nakajima, Amanda C. Richardson, Jolene J. Windle, Hisashi Harada
(A) MEFs were treated with 50 nM of paclitaxel for 48 hrs. Cell death was determined by DNA fragmentation using the Cell Death Detection ELISAplus kit (Roche). Average values from triplicate samples are shown as representative of two independent experiments. (B) MEFs were treated with 50 nM of paclitaxel for the indicated times. Total cell extracts were subjected to Western blotting with the indicated antibodies.