Analysis of rLiEndoG activity on double-stranded DNA.

1 µg of supercoiled plasmid DNA (sc) was digested with increasing amounts of rLiEndoG (0, 20, 40, 80 and 160 ng) and with 160 ng of denatured rLiEndoG protein (160DP) as a negative control. The restriction enzyme Bgl II (Bgl II) was used as a control to generate linear DNA (lin). Each sample of digested DNA was split into two aliquots and one of them was heat-denatured (D) while the other one remained non-denatured (ND). All samples were electrophoresed in a 1% agarose gel and visualized under UV light. The different forms of plasmid DNA are indicated: open circular (oc); linear double-stranded (lin); supercoiled (sc); linear single-stranded (lin ss); circular single-stranded (cir ss).