Fig_1.tif (1.69 MB)
Analysis of LPS expression and immunoreactivity.
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posted on 2015-07-06, 03:53 authored by Dina A. Moustafa, Jennifer M. Scarff, Preston P. Garcia, Sara K. B. Cassidy, Antonio DiGiandomenico, David M. Waag, Thomas J. Inzana, Joanna B. Goldberg(A) LPS extracted from B. mallei (Lane 2), SL3261 (Lane 3), and SL3261/p1C3 (Lane 4) was subjected to SDS-PAGE followed by immunoblotting analysis using 5C8-1C3 anti-B. mallei-specific LPS monoclonal antibody (mAb). Molecular weight marker is shown in Lane 1. (B) LPS extracted from B. mallei (B.m.) and B. pseudomallei (B.p) was run, subjected to SDS-PAGE followed by immunoblot using sera collected from SL3261 (vector)-immunized mice; 3B3-5, anti-B. pseudomallei mAb; sera collected from SL3261/p1C3 (vaccine)-immunized mice; and 5C8-1C3, anti-B. mallei mAb. Molecular weight ladder (L).
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biothreat Burkholderia sppBurkholderia mallei LPS O Antigen Provides ProtectionBurkholderia challengeglandersmimics melioidosispseudomallei LPSSalmonella enterica serovar Typhimurium SL 3261Previous studiesmallei O antigenmurine modelimmunodominant antigenvaccineEtiologic AgentsRecombinant Salmonellacategory B priority pathogenspseudomallei O antigenGlanders Burkholderia pseudomalleiBurkholderia thailandensisBurkholderia malleiAntibody response
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