Analysis of Autophagy in HSV-1 Infection of HeLa Cells.

A. HeLa cells were uninfected (mock), or infected with different strains of HSV-1 (KOS or McKrae). At 2 hpi, the cells were harvested, lysed, and the lysate was immunoblotted for LC3 levels. B. Uninfected or infected HeLa cells were harvested at 6 hpi, lysed, and immunoblotted. C. Immunoblotting of Uninfected or infected HeLa cells at 12 hpi. D. Immunoblotting of Uninfected or infected HeLa cells at 30 hpi. E. Quantification of LC3-II levels in (A-D), normalized to the housekeeping protein GAPDH.