Altered gene expression in Henmt1^PIN/PIN spermatocytes and round spermatids.

(A) A heatmap showing log2 fold change in differentially expressed genes in spermatocytes and their corresponding log2 fold change in round spermatids (round). Color coding represent log2 fold changes of genes. Prm1, Tnp1, Tnp2, Prm2 indicate the position of transcripts subjected to further analysis. (B) A volcano plot showing expression changes of all detected genes in spermatocytes and round spermatids. Dots in cyan represent significantly differential expressed genes (FDR < 0.05) based on edgeR. Fold changes and p-values were calculated with edgeR. (C-G) qPCR of spermiogenic genes including (C) Tnp1 (D) Tnp2 (E) Prm1 (F) Prm2 (G) Gapdhs in 28 day-old Henmt1^WT/WT and Henmt1^PIN/PIN spermatocytes and round spermatids (n = 5 / genotype +/- SEM, * p<0.05, **p<0.01). White bar represents Henmt1^WT/WT and black bar is Henmt1^PIN/PIN. S’cytes = spermatocytes, S’tids = round spermatids. A two-tailed unpaired student T-test was performed for statistical analyses.