Fig_6.tif (777.77 kB)
Agarose gel of PCR amplicons from VPAHPND using the AP3 method.
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posted on 2015-05-27, 04:03 authored by Ratchanok Sirikharin, Suparat Taengchaiyaphum, Piyachat Sanguanrut, Thanh Duong Chi, Rapeepat Mavichak, Porranee Proespraiwong, Bunlung Nuangsaeng, Siripong Thitamadee, Timothy W. Flegel, Kallaya SritunyalucksanaLane M; DNA marker, Lane N: negative control; Lanes 1–3: Positive amplicons (333 bp) from 3 isolates of VPAHPND bacteria; Lanes 4–10: No amplicons from 8 non-AHPND bacteria; Lane P: positive control (333 bp).
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parahaemolyticus M 0605similarity51 VPAHPNDcontig 034 GenBank accessionhepatopancreatic necrosis diseasegavage bioassaysmarker levelsToxA geneammonium sulfate precipitationcause AHPND pathologybroth cultureVibrio parahaemolyticus IsolatesMASCOT analysishepatopancreatic tubule epithelial cellsVibrio parahaemolyticusJALLtubule epithelial cellsPCR detectionnematode bacterium Photorhabdus luminescensgavage assaysshrimp pondsPCR methodbroth cultures16 kDavivo tests50 kDashrimp stomachmass spectrometry analysisPhotorhabdus insectprotein fractionshistological analysisCause Acute Hepatopancreatic Necrosis Diseaseinsecticidal toxins
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