A simplified scheme of the glycerol assimilation pathway in yeast.

Glycerol kinase 1 (Gut1p) knocked out in this study is involved in the first step of glycerol metabolism. Gpp1p and Gpp2p: glycerol-3-phosphatases 1 and 2, Gpd1p and Gpd2p: glycerol 3-phosphate dehydrogenases 1 and 2, Dak1 and Dak2p: dihydroxyacetone kinases 1 and 2, Gcy1p: putative NADP⁽⁺⁾ coupled glycerol dehydrogenase, DHA: dihydroxyacetone, DHAP: dihydroxyacetone phosphate, G3P: Glycerol-3-phosphate, “?”: the enzyme responsible for the conversion of DHAP to DHA is not verified. Under aerobic conditions, mitochondrial Gut2p (glycerol-3-phosphate dehydrogenase 2) can convert G3P to DHAP. Figure modified from [59].