5-FU-resistant clones display morphological changes that are associated with EMT.

(A) Phase contrast photos showing cell morphology of normal (Nw, Nt) and chemoresistant clones (B1Q, B1V), 40x. (B-D) RNA levels (relative quantity) of vimentin, E-cadherin, and N-cadherin, as measured by RT-PCR. Error bars represent standard deviation. Statistically significant difference between the chemosensitive and the chemoresistant clones (P <0.05) is indicated by *. (E-G) Western blot and immunostain showing the levels of vimentin, E-cadherin, and N-cadherin, in the chemosensitive (Nt, Nw) and the chemoresistant (B1Q, B1V) cell lines. (H) Immunostain and (I) Western blot showing levels of CD19 in the chemosensitive and the chemoresistant cell lines. (J) Graph displaying the results of 24 hour invasion assays performed on the clones, with phase contrast images (20x) showing representative areas of invading cells stained with crystal violet stain (K). Assay was performed three times. Statistically significant difference between the chemosensitive and the chemoresistant clones (P <0.05) is indicated by *.