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2DE proteomic analysis.

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posted on 2009-12-02, 01:01 authored by Manuela Basso, Giuseppina Samengo, Giovanni Nardo, Tania Massignan, Giuseppina D'Alessandro, Silvia Tartari, Lavinia Cantoni, Marianna Marino, Cristina Cheroni, Silvia De Biasi, Maria Teresa Giordana, Michael J. Strong, Alvaro G. Estevez, Mario Salmona, Caterina Bendotti, Valentina Bonetto

Representative Sypro Ruby-stained 2DE maps of TIF of late-symptomatic G93A SOD1 mice (A) and age-matched WT SOD1 mice (B). In panel A the numbered spots correspond to proteins enriched or only present in TIF of G93A samples, and in panel B they indicate proteins enriched in TIF of WT samples. The same amount of protein was loaded in each gel (75 µg). The asterisk indicates the spot corresponding to GFAP, which is the most prominent, but equally abundant in the two conditions, and was therefore considered as background.

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