O cultures 2 × 10beads/ml of fluorescent polystyrene beads as described in Methods. -, confocal microscopy images, showing the beads uptake (yellow-green hue) in control (, ), LPS treated (, ) and Otreated MDMs (-); cells were counter-stained with 1 μg/ml propidium iodide (red hue). , , , Original magnification: 40×; , , , , original magnification: 100×. , , Evaluation of phagocytic activity performed counting the number of phagocytic MDMs, reported as percent of phagocytic cells (solid bars), as well as the number of beads per cell (■). A minimum of 500 cells per sample were observed. Evaluation of percentage of phagocytic cells in controls maintained at 0°C, to block beads internalization, is also reported (yellow solid bars).
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Taken from "Stimulatory effect of essential oil on innate cell-mediated immune response"