tbsp_a_946877_sm7438.pdf (532.8 kB)
Proteins immobilization on the surface of modified plant viral particles coated with hydrophobic polycations
Version 3 2014-10-01, 08:43
Version 2 2014-10-01, 08:43
Version 1 2014-10-01, 08:43
journal contribution
posted on 2014-10-01, 08:43 authored by Nikolai A. Nikitin, Andrei S. Malinin, Ekaterina A. Trifonova, Anna A. Rakhnyanskaya, Aleksandr A. Yaroslavov, Olga V. Karpova, Joseph G. AtabekovTwo hydrophobic cations based on poly-N-ethyl-vinylpyridine were used to produce biologically active complexes. The complexes obtained from tobacco mosaic virus (TMV) spherical particles (SPs), hydrophobic polycation, and a model protein were stable and did not aggregate in solution, particularly at high ionic strengths. The nucleic acid-free SPs were generated by thermal remodeling of the TMV (helical rod-shaped plant virus). The model protein preserved its antigenic activity in the ternary complex (SP–polycation–protein). Immobilization of proteins on the surface of SPs coated with hydrophobic cation is a promising approach to designing biologically active complexes used in bionanotechnologies.