MALDI Imaging Mass Spectrometry of a Mouse Kidney

Instrument: Autoflex III Smartbeam (Bruker, Bremen, Germany)

Linear mode (m/z 2000 - 20000)

Negative ion mode

Matrix: 2,4,6-Trihydroxyacetophenone

Spatial resolution: 150 µm

Tissue

- Mouse Kidney (BI/6, C57BL/6JRccHsd)

- Healthy

- Snap frozen in liquid N2 immediately after harvest

- 12 µm section (on Leica cryostat @ - 30°C)

 

Matrix application

Sublimation @ 160°C for 12 min 30 sec

 

Recrystallization

With 1 mL of 5% MeOH, 5% Acetic Acid @ 80°C for 2 min 30 sec (starting from RT)

 

Tissue wash

According to Yang and Caprioli, Anal. Chem. 2011, 83, 5728-5734 ; Rinse protocol D