Col-HA defect images

Data associated with article:

Effects of cell-attachment and extracellular matrix on bone formation in vivo in collagen-hydroxyapatite scaffolds

Max M. Villa, Liping Wang, David W. Rowe, and Mei Wei

Department of Materials Science & Engineering, 97 North Eagleville Road, University of Connecticut, Storrs, CT 06269

Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030

This dataset contains histological sections of critical size calvarial defects filled with collagen-hydroxyapatite scaffolds and culture expanded bone marrow osteoprogenitors after three weeks in vivo.

Naming scheme:

Group_AnimalEarNotch-Slide#_replicate#Channel#+Channel#+Channel#.jpg

example:
G1-NB-1_s1c2.jpg

Explanation:
Group
G1 - samples incubated in vitro overnight before implantation
G2 - samples incubated in vitro overnight before implantation +BME gel used as a seeding suspension
G3 -samples implanted immediately after seeding
G4 -samples implanted immediately after seeding +BME gel used as a seeding suspension

AnimalEarNotch
G1&G2
NB - ear notch both
NL - ear notch left
NR - ear notch right
HR - hole right
HL - hole left
HB - hole both
G3&G4
NB - ear notch both
NL - ear notch left
NR - ear notch right
HR - hole right
HL - hole left
HB - hole both
Slide Number
Three tissue sections were placed on a slide. Slide number can be a value 1, 2 or 3.
Replicate number
One the replicate serial sections of the same calvarium, can be a value s1, s2 or s3.
Channel number
Corresponds to 1 of the 3 channels acquired:
Channel1-c1- DIC, grayscale
Channel2-c2- calcein mineralization label, green
Channel3-c3- Tdtomato, donor cells, red