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Biotinylated fluorophosphonate (FP-biotin) labelling of fibroblast activation protein alpha (FAPα) on chondrocyte membranes
figure
posted on 2011-12-31, 02:05 authored by Jennifer M Milner, Lara Kevorkian, David A Young, Debra Jones, Robin Wait, Simon T Donell, Emma Barksby, Angela M Patterson, Jim Middleton, Benjamin F Cravatt, Ian M Clark, Andrew D Rowan, Timothy E CawstonCopyright information:
Taken from "Fibroblast activation protein alpha is expressed by chondrocytes following a pro-inflammatory stimulus and is elevated in osteoarthritis"
Arthritis Research & Therapy 2006;8(1):R23-R23.
Published online 3 Jan 2006
PMCID:PMC1526559.
Membrane extracts isolated from IL-1 plus oncostatin M stimulated chondrocytes were treated with or without FP-biotin. Labelled proteinases were isolated using streptavidin-agarose beads and eluted with reducing SDS-PAGE loading buffer. Proteins were separated by SDS-PAGE and stained with colloidal Coomassie. The 97 kDa protein was identified by mass spectrometry to be FAPα.