figshare
Browse

Dissimilatory sulphate reduction in archaeon Candidatus Vulcanisaeta moutnovskia sheds light on evolution of sulphur metabolism

Download all (572.49 kB) This item is shared privately
dataset
modified on 2022-09-28, 09:54
Data and code that accompanies our article which is submitted to Nature Microbiology and is entitled: Dissimilatory sulphate reduction in archaeon Candidatus Vulcanisaeta moutnovskia sheds light on evolution of sulphur metabolism



Authors: Nikolay A. Chernyh1*, Sinje Neukirchen2, Evgenii N. Frolov1, Filipa L. Sousa2*, Margarita L. Miroshnichenko1, Alexander Y. Merkel1, Nikolay V. Pimenov1, Dimitry Y. Sorokin1, Sergio Ciordia3, María Carmen Mena3, Manuel Ferrer4, Peter N. Golyshin5, Alexander V. Lebedinsky1, Inês A. Cardoso Pereira6*, Elizaveta A. Bonch-Osmolovskaya1,7


1 Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology, Russian Academy of Sciences, Leninsky prospect 33 Bldg 2, 119071, Moscow, Russia
2 Archaea Biology and Ecogenomics Unit, Department of Functional and Evolutionary Ecology, University of Vienna, Althanstrasse 14 UZA I, 1090 Vienna, Austria
3 Proteomics Facility, Centro Nacional de Biotecnología, CSIC, Madrid, Spain
4 Institute of Catalysis, CSIC, Madrid, Spain
5 School of Biological Sciences, Bangor University, Gwynedd LL57 2UW, UK
6 Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
7 Lomonosov Moscow State University, Faculty of Biology, 1-12 Leninskie Gory, 119991, Moscow, Russia.

*Corresponding authors: chernyh3@yandex.com, filipa.sousa@univie.ac.at, ipereira@itqb.unl.pt

We provide one compressed file containing the supplementary data files for Figures 3 and 5 and Extended Data Figures 8-10 (Data_trees_alignments.zip). In addition, we also provide the small scripts to aid in the reproduction of the analysis (scripts.zip).

Funding

This work was supported in part by grant # 1774-30025 of the Russian Science Foundation, Program “Molecular and Cell Biology” of the Russian Academy of Sciences, as well as grant # 13-04-01695 of the Russian Foundation for Basic Research and grant of the Ministry of Science and Higher Education of the Russian Federation (to N.A.C., A.V.L., E.N.F., M.L.M., A.Y.M., N.V.P. and E.A.B-O). Sequencing of PCR amplicons was performed using the scientific equipment of the Core research facility “Bioengineering” by Tatyana Kolganova. The proteomic analysis was performed in the Proteomics Facility of The Spanish National Center for Biotechnology (CNB-CSIC), which belongs to ProteoRed, PRB2-ISCIII, supported by grant PT13/0001 (to S.C., M.C.M., M.F.). P.N.G. acknowledges the funding of the UK Biotechnology and Biological Sciences Research Council (BBSRC) within the ERA NET-IB2 program, grant number ERA-IB-14-030 and European Union Horizon 2020 research and innovation program [Blue Growth: Unlocking the potential of Seas and Oceans] under grant agreement No [634486] and the support of the Centre of Environmental Biotechnology (CEB) Project part-funded by the European Regional Development Fund (ERDF) through the Welsh Government and the support of the Centre of Environmental Biotechnology (CEB). D.Y.S. was supported by the SIAM/Gravitation Program (Dutch Ministry of Education and Science, grant 24002002) and RFBR grant 19-04-00401. F.L.S. and S.N. acknowledge support from the Wiener Wissenschafts, Forschungs- und Technologiefonds (Austria) through the grant VRG15-007. F.L.S. gratefully acknowledges funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement 803768). I.A.C.P. acknowledges support from the Fundação para a Ciência e Tecnologia (Portugal) through grant PTDC/BIA-BQM/29118/2017 and R&D unit MOSTMICRO-ITQB (UIDB/04612/2020 and UIDP/04612/2020).

    Categories

    Keywords

    dissimilatory sulphate reductionArchaeaMicrobiology

    Licence

    CC BY 4.0