V4 16S sequencing of batch cultured vaginal microbiota in prebiotics

Vaginal microbiota from 4 subjects (2N, BZ, C9, N6) were collectively grown in vaginally-defined medium without glucose (o), or with 0.5% glucose (G), lactitol (tol), lactulose (lose), raffinose (R) or oligofructose (OF). Time points taken for V4 variable region of 16S rRNA sequencing by Illumina MiSeq were 0, 6, 12, 24, and 48 hours after inoculation.