Absorption

The absorbance spectra from 28 trials per stiff fraction per TBHP treatment with independently stained platelets were loaded onto multiple 96-well plates. Each well contained a total volume of 100 microliter of reconstituted whole blood with 30% tank hematocrit, which corresponds to a sample path length of 3.108 mm. The plates were measured using a Synergy H1 - BioTek microplate reader. The absorbance spectra was analyzed over a range of wavelengths from 600 to 700nm, including the peak emission of the platelet fluorescence (660nm). An ipython jupyter notebook is included to display the data.