VIDEO 2 Polusani etal

1.      VIDEO 2 – Homotypic (top two panels) or Mixed (bottom panel) confluent cultures of HeLa26 (green) and HeLa43 (red) were subjected to a scrape wound and followed as in Video 1, but with longer spacing between frames. Cx26 and Cx43 are known not to form heterotypic channels, so these two cell lines do not couple. Each cell type was labeled by a lipohilic dye (DiI (Cx43) and DiO (Cx26), and the color distribution in each cell averaged by Metamorph software. In the mixed culture (lower panel) the green cells (HeLa26) fill the wound more rapidly than the red ones (HeLa43), each migrating at rates similar to what they do in homotypic cultures (shown in panels above). Quantitation shown in Fig 3B