VIDEO 1 Polusani et al wound healing HeLa par and 26.mov

1.      VIDEO 1 – HeLa parental and HeLa26 confluent cultures were subjected to a scrape wound and allowed to close the wound over 28 hours. The timelapse video shows how the HeLa parental cells close the wound slowly by progressing into the wounded area as a cohesive “sheet”, maintaining close cell contact. In contract, the HeLa cells expressing Cx26 move more quickly to fill the wound, frequently breaking contact with the main monolayer and actively migrating independently, showing much higher rates of filopodial extension and activity. Quantification of motility rates shown in Fig 1