Movies S1.A-F

Movies used in manuscript " Cellular glycosaminoglycans and viral protein glycosylation regulate diffusion and entry of herpesvirus at cell surface "

Movies S1. Timelapses of HSV-1 (KOS strain) diffusing at the surface of (A) wt CHO-K1 cells expressing both herapan sulfate (HS) and chondroitin sulfate (CS), (B) wt CHO cells treated with chondroitinase ABC, lacking chondroitin sulfate (CHO-ΔCS), (C) pgsD-677 lacking HS (CHO-ΔHS). (D) Timelapse of HSV-1 (KOS strain) on wt HaCat cells. (E) Timelapse of the mutant HSV-1 (KOS-gCΔmuc) on wt HaCat cells. The scale bar represents 10 μm. Using Fiji, all movies were corrected for uneven background using the same procedure with a rolling ball of 50 and the noise was despeckled. The LUT thresholds were set to be 10 as minimum and 50 as maximum for all videos. Finally, the movies were then saved as .avi using an acceleration of 50 frame per second. (F) The virus trajectories reconstructed using Trackmate are shown on the movie corresponding to A for wt CHO. The colors of the trajectory are arbitrary.