Figure S13. Isolated membrane protrusions do not trigger dynamin2 recruitment.

(A) L. monocytogenes membrane protrusions were isolated from infected HeLa cells that were labelled with CellTracker Blue (blue). The isolated protrusions were overlaid onto naïve cells expressing dynamin2-mCherry (“Dyn2-mCherry, red). Alexa 488-phalloidin (green) was used to visualize the F-actin within the protrusions. Solid arrowheads indicate caveolin-1-mCherry encapsulating the entire isolated protrusion. Open arrowheads indicate the bacterium inside the protrusion. Scale bar = 2μm.

(B) Line scan analysis of (A). A single 1.5 µm line (white line) was drawn across the isolated protrusion and F-actin intensity as well as the corresponding dynamin2-mCherry and CellTracker Blue intensity was plotted.