Supplemental Figure S1.tif

Supplemental Figure S1. Phenotypic characterization of late EPCs. (A) Sequential changes of cultured EPC. a, Peripheral blood mononuclear cells immediately after plating. b, One day after seeding. c, Three days after plating. d, Seven days after plating. e, Ten days after plating. f, Two weeks after plating. g, Late EPC grew exponentially. h, Late EPC grown to confluence showing a cobblestone-like monolayer (Scale bar=100 μm). (B) After 28 days cultured, all cultured EPCs were dually positive for DiI-acLDL uptake and FITC-labeled BS-1 lectin binding (Scale bar=75 μm). (C) Flow cytometry analysis of marker proteins CD34, CD45, KDR, CD31, and VE-cadherin of cultured late EPCs from healthy and hypertension patients. (n=5 per group) (Hypertens.=hypertension).