Droplet-based, high-throughput single cell transcriptional analysis of adult mouse tissue using 10X Genomics' Chromium Single Cell 3' (v2) system: From tissue preparation to bioinformatic analysis
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modified on 2019-03-06, 21:06 The attached R Scripts supplement our protocol paper currently under editorial review at the Journal of Visualized Experiments.
Scope of the article:
This protocol describes the general processes and quality control checks necessary for preparing healthy adult single cells in preparation for droplet-based, high-throughput single cell RNA-Seq analysis using the 10X Genomics' Chromium System. We also describe sequencing parameters, alignment and downstream single-cell bioinformatic analysis.
