figshare
Browse
Unigene.fasta (55.91 MB)

Unigenes of passion fruit chilling stress

Download (55.91 MB) This item is shared privately
dataset
modified on 2020-12-27, 03:17

Chilling stress (CS) is an important limiting factor for the growth and development of passion fruit (Passiflora edulis) in winter in south China. However, little is known about how the passion fruit responds and adapts to CS. In this study, we performed transcriptome sequencing of Huangjinguo (HJG, cold-susceptible) and Tainong 1 (TN1, cold-tolerant) under normal temperature (NT) and CS conditions, and a total of 47,353 unigenes were obtained in 7 databases. Using differentially expressed unigenes (DEGs) analysis, 3,248 and 4,340 DEGswere identified at two stages, respectively. The Gene Ontology (GO) enrichment analysis showed that the DEGs were mainly related to phosphorylation, membrane protein, and catalytic activity. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, the unigenes of plant-pathogen interaction, plant hormone signal transduction and fatty acid metabolism were enriched.Then, the 12,471 filtered unigenes were divided into 8 co-expression modules, and two of which were correlated with CS. In the two modules, 32 hub unigenes were obtained. Furthermore, the unigenes related to CS were validated using quantitative real-time PCR (RT-qPCR). This work showed that the expression levels of CS-related unigenes were very different in two passion fruit cultivars. The results provide information for the development of passion fruit with increased chilling tolerance.