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Tenofovir resistance in HBV

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modified on 2020-06-15, 08:04
These files are the supporting data for a project to identify mutations in Hepatitis B Virus (HBV) that have been reported in association with resistance to tenofovir.

Suppl Fig 1: Schematic view of Pharmacodynamics of TDF, TAF and ADV.

Suppl Fig 2: PRISMA flow diagram to illustrate the identification of studies for inclusion in a systematic review of tenofovir resistance in HBV infection (provided in pdf format).

Suppl Table 1: Metadata for 15 studies reporting TDF resistance in HBV infection identified from a systematic literature review.

Suppl Table 2: Quality assessment for 15 studies reporting TDF resistance in HBV infection. Studies identified from a systematic literature review.

Suppl Table 3: List of 37 tenofovir RAMs in HBV Reverse Transcriptase (RT) reported in 15 studies identified through a systematic literature review (provided in xls format).

Suppl Table 4: List of TFV RAMs in HBV, showing consensus residue at each position according to HBV genotype, and genotype-specific resistance where this has been reported. List of RAMs obtained from 12 studies included in the review. HBV reference sequences used are the same as for Fig 1. Grey colour highlights the sites that differ from majority consensus. Ticks represent RAMs situated in sites where there is a difference (at consensus level) between genotype sequences. RAMs are listed with the wild-type amino acid first, followed by the numbered position in RT sequence, and the amino acid substitution that is associated with resistance. TFV = tenofovir, RAMs = resistance associated mutations, HBV = hepatitis B virus.

Suppl Table 5: Reference sequence alignment of HBV RT and HIV RT. Sequences downloaded from HIV sequence database online at https://www.hiv.lanl.gov/ and Hepatitis B Virus Database online at https://hbvdb.ibcp.fr/HBVdb/. Alignment undertaken using MAFFT version 7. HIV subtype B reference sequence is shown in light green (accession number K03455). HBV reference sequences (Geno-A: FJ692557; Geno-B: GU815637; Geno-C: GQ377617; Geno-D: KC875277; Geno-: GQ161817 shown in light yellow. TFV RAMs are highlighted in red. HIV TFV RAMs were obtained from Stanford Database (https://hivdb.stanford.edu/dr-summary/resistance-notes/NRTI/). HBV TFV RAMs were obtained from 12 studies included in the review. Sites with the same amino acid at homologous sites are indicated with asterisks (*); among these sites, those that are also shared TVF RAMs are shaded in light blue.

Suppl Table 6: Mapping of RAMs to the HIV RT structure. RAMs were mapped onto the high-resolution structure of HIV RT to infer their possible functional consequences in terms of structure.

PRISMA checklist: criteria for a systematic review

Funding

Wellcome (ref 110110)