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Single Cell RNAseq DGE tables for Fetal and Postnatal Dataset from Smith et al, 2021. PNAS
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modified on 2021-07-26, 16:13 To gain cell-biological insights into the spatiotemporal dynamics of prenatal ATP1A3 expression, we established a transcriptional atlas of ATP1A3 expression during cortical development using mRNA in situ
hybridization and transcriptomic profiling of ~125,000 individual cells
with single-cell RNA sequencing (Drop-Seq) from various areas of the
midgestational human neocortex. We find that fetal expression of ATP1A3
is restricted to a subset of excitatory neurons carrying
transcriptional signatures of neuronal activity and maturation
characteristic of the developing subplate. Furthermore, by performing
Drop-Seq on ~52,000 nuclei from four different areas of an infant human
neocortex, we show that ATP1A3 expression persists throughout
early postnatal development, not only within excitatory neurons across
all cortical layers, but also and more predominantly in inhibitory
neurons, with specific enrichment in fast-spiking basket cells. In
addition, we show that ATP1A3 expression, both in fetal and
postnatal neurons, tends to be higher in frontal cortical areas than in
occipital areas, in a pattern consistent with the rostro-caudal
maturation gradient of the human neocortex.
Funding
Role for ion conducting proteins in cortical malformation diseases
National Institute of Neurological Disorders and Stroke
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