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STABILITY OF THE HUMAN GUT VIROME AND EFFECT OF GLUTEN-FREE DIET

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modified on 2024-02-08, 11:50

The human gut microbiome consists of bacteria, archaea, eukaryotes, and viruses. The gut viruses are relatively underexplored. Here, we longitudinally analysed the gut virome composition in 11 healthy adults, its stability, variation, and the effect of a gluten-free diet. Using viral enrichment and a de novo assembly-based approach, we demonstrate the quantitative dynamics of the gut virome, including dsDNA, ssDNA, dsRNA, and ssRNA viruses. We observe highly divergent individual viral communities, carrying on an average 2,143 viral genomes, 13.1% of which were present at all three time points. In contrast to previous reports, the Siphoviridae family dominates over Microviridae in studied individual viromes. We also show individual viromes to be stable at the family level but vary substantially at the genera and species levels. Finally, we demonstrate that lower initial diversity of the human gut virome leads to a more pronounced effect of the dietary intervention on its composition.

Funding

NWO - Dutch Research Council (NWO Dutch: Nederlandse Organisatie voor Wetenschappelijk Onderzoek) Vidi (NWO-VIDI 016.178.056)

ERC starting grant (ERC Starting Grant 715772)

NWO Gravitation grant Exposome-NL (024.004.017)

NWO Vidi (NWO-VIDI 864.13.013)

CardioVasculair Onderzoek Nederland (CVON 2018–27) grant

ERC advanced grant (FP/2007–2013/ ERC grant 2012–322698)

NWO Spinoza prize (NWO SPI 92–266)

NWO Gravitation Netherlands Organ-on-Chip Initiative (024.003.001)

NWO Vidi grant 864.14.004

ERC Consolidator grant 865694: DiversiPHI

SFI/12/RC/2273

scholarship from the Graduate School of Medical Sciences, University of Groningen

scholarship from the Junior Scientific Masterclass, University of Groningen

ERC Consolidator grant 101001678

NWO-VICI grant VI.C.202.022

SFI-HRB-Wellcome Trust Research Career Development Fellowship #220646/Z/20/Z