Analyses of gene expression data from the microglial RNA of uninfected and P. berghei-infected wild-type C57BL/6 and IFNAR knock-out mice

The C57BL/6 mouse infected with Plasmodium berghei ANKA is a common experimental model for cerebral malaria. In order to investigate the effect on microglial cells of Type 1 interferon signalling during experimental cerebral malaria infection, transcriptomes of naive and P. berghei-infected wild-type (WT) C57BL/6 and interferon-α/β receptor knock-out (IFNAR-KO) mice were generated. These transcriptomic data has been deposited at NCBI Gene Expression Omnibus (GEO) under accession number GSE119650.

This dataset presents analyses of the raw transcriptomic data available from GEO. All data are supplied either as PDF or tab-delimited text files. Files available are as follows:

PCA.pdf - Principle Component Analysis of the top 500 genes showing variance across the naive and infected WT and IFNAR-KO samples.

wt.inf_vs_wt.naive-p01.fc2.results.txt - Differential gene expression analysis of naive and infected WT microglial cells.

ko.inf_vs_ko.naive-p01.fc2.results.txt - Differential gene expression analysis of naive and infected IFNAR-KO microglial cells.

ToppGene.enrichment.barplot.pdf - A bar plot comparing hits to the Reactome Pathway Database derived from gene list enrichment analysis of the top 500 unique Entrez gene identifiers from each differential gene expression analysis.

ko_specific_treatment_effect.heatmap.pdf - A heatmap indicating genes that displayed a IFNAR-KO-specific response to P. berghei infection versus WT microglial cells.