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SLCA screen of interactions between 279 tomato kinases and five effectors from the model tomato pathogen, Pseudomonas syringae pv. tomato (Pst) including HopA1, HopAI1, HopAF1, AvrPto, and HopM1. 1,170 pairwise kinase–effector (K-E) interactions were tested in tomato protoplasts using the split luciferase complementation assay (SLCA) where luciferase activity indicates reconstitution of the N-terminal and C-terminal domains of the enzyme fused to interacting bait or prey proteins.


National Science Foundation award IOS-1025642