Macro1 for single images.ijm (5.29 kB)

Macro 1

Download (5.29 kB) This item is shared privately
modified on 16.05.2017, 18:28
The macro analyses folders of phase-contrast microscope images of multicellular spheroids and returns spheroid area, min and max Ferret diameter and a few other features. It uses a number of filters trying to clean up the image from scratches in the plate, bubbles and the odd fiber. The macro also records a quality control image with the overlay of the spheroid(s) it has recognized and sized in the output folder. 

Researchers interested in three-dimensional cell culture and especially hanging-drops or overlay spheroid cultures can take series of images of their spheroids and the macro will automate the process of sizing cultures.


Delyan Ivanov was supported by an EPSRC Doctoral Prize award hosted by the University of Nottingham (DP2014/DI)