Primer sequences and conditions for PCR amplification for COI barcode of Cimex, presence of Wolbachia, presence of Symbiopectobacterium, and presence of Ca. Tisiphia

Column 1: Primer pair name

Column 2 : Primer Sequence (5'- 3')

Column 3: Target organism

Column 4: Target Gene

Column 5: Ta (^oC) (annealing temperature in PCR profile)

Column 6: Expected amplicon size (bp)

Column 7:References

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Pilgrim J, Ander M, Garros C, Baylis M, Hurst GDD, Siozios S. Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features. Environ Microbiol. 2017;19(10):4238-55

Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America. 1994;87(6):651-701.