fission yeast structural variation
Published on by Daniel Jeffares
Large structural variations (SVs) in the genome are harder to identify than smaller genetic variants but may substantially contribute to phenotypic diversity and evolution. Here we analyse the effects of SVs on gene expression, quantitative traits, and intrinsic reproductive isolation in the yeast Schizosaccharomyces pombe. We establish a high-quality curated catalog of SVs in the genomes of a worldwide sample of S. pombe strains, including duplications, deletions, inversions and translocations. We show that copy number variants (CNVs) frequently segregate within closely related clonal populations and are in weak linkage with single nucleotide polymorphisms (SNPs), indicating rapid turnover. We show that these transient CNVs produce stoichiometric effects on gene expression both within duplicated regions and in other genomic regions. CNVs make substantial contributions to quantitative traits such as cell shape, cell growth under diverse conditions, sugar utilisation in winemaking.
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Wellcome Trust 095598/Z/11/Z