FAST demonstration data

Example datasets to illustrate the capabilities of FAST, the Feature-Assisted Segmenter/Tracker.

Consists of three folders:

1. Slingshots

Contains images of P. aeruginosa cells in a high-density monolayer, moving via twitching motility. Images were collected at high temporal resolution - approximately 127 fps - using camera streaming. Track data indicates low-frequency, ultra-rapid movements by individual cells.

2. Lineages

Contains images of E. coli cells dividing to form a microcolony, confined to two dimensions in a polyacrylamide microfluidic device. Track data reconstructs the formation of lineages from the two founder cells.

The underlying dataset was extracted from the supplementary data files of 'Microfabricated Polyacrylamide Devices for the Controlled Culture of Growing Cells and Developing Organisms', released under the Creative Commons Attribution License.

3. T6SS

Contains four datasets, consisting of high-density co-cultures of P. aeruginosa and V. cholerae under agarose pads. Cells contained fluorescently-tagged sheath proteins of the Type 6 Secretion System (T6SS). Track data allows quantification of firing rates under different condtions.

The underlying datasets were extracted from the supplementary data files of 'The evolution of tit-for-tat in bacteria via the type VI secretion system', released under the Creative Commons Attribution License.

Further information

The outputs of these analyses are presented along with explanations in the manuscript 'Tracking bacteria at high density with FAST, the Feature-Assisted Segmenter/Tracker'.

For further details about how these files are formatted, please refer to the project wiki.