Conformation of the nuclear pore in living cells is modulated by transport state

Data from: Conformation of the nuclear pore in living cells is modulated by transport state Authors: Joan Pulupa1, Harriet Prior1, Daniel S. Johnson2, Sanford M. Simon1,*. Affiliations: 1Laboratory of Cellular Biophysics, Rockefeller University, New York, NY 10065, USA. 2Department of Physics and Astronomy, Hofstra University, Hempstead, NY 11549, USA. Abstract: While the static structure of the nuclear pore complex (NPC) continues to be refined with cryo-EM and x-ray crystallography, in vivo conformational changes of the NPC remain under-explored. We developed sensors that report on the orientation of NPC components by rigidly conjugating mEGFP to different NPC proteins. Our studies show conformational changes to select domains of Nups within the inner ring (Nup54, Nup58, Nup62) when transport through the NPC is perturbed and no conformational changes to Nups elsewhere in the NPC. Our results suggest that select components of the NPC are flexible and undergo conformational changes upon engaging with cargo.