The attached data files are companions to the manuscript “A genetic screen suggests an alternative mechanism for inhibition of SecA by azide” by Jamshad et al. at BiorXiv (manuscript ID BIORXIV/2017/173039). Below is a description of the files. A full description of the methods can be found in the associated manuscript. sequence.gb : NCBI file for Escherichia coli K-12 strain W3110 (AP009048) containing genome sequence and feature annotations. rclb_genisect.bed and rclb_genisect_2.bed : BED files of processed sequences of the TraDIS library after growth in LB in the absence of sodium azide aligned to AP009048. rc0.25_genisect.bed and rc0.25_genisect_2.bed : BED files of processed sequences of the TraDIS library after growth in LB in the presence of 0.25 mM sodium azide aligned to AP009048. rc0.5_genisect.bed and rc0.5_genisect_2.bed : BED files of processed sequences of the TraDIS library after growth in LB in the presence of 0.5 mM sodium azide aligned to AP009048.