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\ p None B a = c @ = Z ?N*8 X" 1 Arial1 Arial1 Arial1 Arial1 Arial1 Arial1 Arial General ` Table_1 7a +
Allele Transgene. In vitro PIP2 binding1 In vivo PIP2 sensitivity0 In vivo motor on synaptic vesicles( Rescue of unc-104 function. UNC-104 Protein levels and localization Wild type High Insensitive (saturated?) ----% High, largely in synapse-rich regions- unc-104(e1265) encodes UNC-104(D1497N) Poor insensitive Very low* Very low, residual in synapse-rich regions9 unc-104(e1265tb107) encodes UNC-104(D1497N R1501Q) Medium sensitive" More than in unc-104(e1265) Medium, residual in synapse-rich9 unc-104(e1265tb120) encodes UNC-104(D1497N M1540I) UNC-104::GFP ------ Full Similar to wild type UNC-104(D1497N)::GFP No No GFP tagged protein observed* UNC-104(D1497N)::GFP very high copy number UNC-104(M1540I)::GFP UNC-104(D1497N M1540I)::GFP Nearly full UNC-104(D1497N R1501Q)::GFP UNC-104(R1501Q)::GFP UNC-104(W1549A)::GFP" UNC-104(D1497N M1540I W1549A)::GFP( UNC-104(KK1463/4AA)::GFP high expression Poor [12] -------' Poorer than UNC-104(D1497N M1540I)::GFP$ UNC-104(R1496A)::GFP high expression
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