Effect of combined MAPK inhibition on long-term melanoma cultures.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Thu Dec 10, 2015
(m / multi-sentence
:snt1 (a / and
:op1 (i / intrigue-01
:ARG0 (d / data
:mod (t / this)))
:op2 (r2 / recommend-01
:ARG1 (v / validate-01
:ARG1 d
:manner (f / further)
:manner (i2 / in-vivo)))
:ARG1-of (d2 / describe-01
:ARG0 (f2 / figure :mod "7")))
:snt2 (a2 / affect-01
:ARG0 (i3 / inhibit-01
:ARG1 (p / pathway
:name (n / name :op1 "MAPK"))
:ARG1-of (c / combine-01))
:ARG1 (c2 / culture-01
:ARG1 (m2 / medical-condition
:name (n2 / name :op1 "melanoma"))
:ARG1-of (l / long-03))))
# ::id a_pmid_2514_2146.254
# ::date 2015-06-14T08:06:35
# ::file a_pmid_2514_2146_254.txt
# ::snt M238 and M792, two MAPK inhibitor-sensitive cell lines, were chronically exposed to 500nM of Vemurafenib (V), SCH722984 (E) or combination (V + E) in 96-well plates.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Sun Jan 17, 2016
(e / expose-01
:ARG1 (a / and
:op1 (c2 / cell-line
:name (n2 / name :op1 "M238"))
:op2 (c3 / cell-line
:name (n3 / name :op1 "M792"))
:ARG0-of (s / sensitive-03
:ARG1 (m / molecular-physical-entity
:ARG0-of (i / inhibit-01
:ARG1 (p / protein-family
:name (n / name :op1 "MAPK"))))))
:ARG2 (o / or
:op1 (s3 / small-molecule
:name (n4 / name :op1 "Vemurafenib")
:quant (c4 / concentration-quantity
:quant "500"
:unit (n5 / nanomolar))
:xref (x / xref :value "PUBCHEM:42611257" :prob "17.762056"))
:op2 (s2 / small-molecule
:name (n6 / name :op1 "SCH722984"))
:op3 (c5 / combine-01
:ARG1 s3
:ARG2 s2))
:manner (c / chronic)
:location (p2 / plate
:quant "96"
:mod (w / well)))
# ::id a_pmid_2514_2146.255
# ::date 2015-06-14T08:12:39
# ::file a_pmid_2514_2146_255.txt
# ::snt A.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Fri Oct 23, 2015
(h / have-li-91 :ARG2 "A")
# ::id a_pmid_2514_2146.256
# ::date 2015-06-14T08:13:07
# ::file a_pmid_2514_2146_256.txt
# ::snt Schematic.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Sun Jun 14, 2015
(s / schematic)
# ::id a_pmid_2514_2146.257
# ::date 2015-06-14T08:14:07
# ::file a_pmid_2514_2146_257.txt
# ::snt For each cell line, 20,000 cells/well were plated on 96-well plates.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Sun Jun 14, 2015
(p / plate-00
:ARG1 (o / or
:op1 (c / cell :quant "20000")
:op2 (w / well :quant "20000"))
:condition (c2 / cell-line
:mod (e / each))
:location (p2 / plate
:quant "96"
:mod (w2 / well)))
# ::id a_pmid_2514_2146.258
# ::date 2015-06-14T08:18:50
# ::file a_pmid_2514_2146_258.txt
# ::snt The following day, all cells on the plate were treated with 500nM of each drug or combination.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Tue Dec 15, 2015
(t / treat-04
:ARG1 (c / cell
:location (p / plate)
:mod (a / all))
:ARG2 (o / or
:op1 (d / drug
:mod (e / each)
:quant (c2 / concentration-quantity
:quant "500"
:unit (n / nanomolar)))
:op2 (c3 / combine-01
:ARG1 d))
:time (d2 / day
:ARG1-of (f / follow-01)))
# ::id a_pmid_2514_2146.259
# ::date 2015-06-14T08:35:34
# ::file a_pmid_2514_2146_259.txt
# ::snt Fresh media containing the appropriate inhibitor(s) was added weekly.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Mon Dec 21, 2015
(a / add-02
:ARG1 (m / medium
:ARG1-of (f / fresh-04)
:ARG0-of (c / contain-01
:ARG1 (m2 / molecular-physical-entity
:ARG0-of (i / inhibit-01)
:ARG1-of (a2 / appropriate-02))))
:frequency (r / rate-entity-91
:ARG1 "1"
:ARG2 (t / temporal-quantity
:quant "1"
:unit (w / week))))
# ::id a_pmid_2514_2146.260
# ::date 2015-06-14T08:42:36
# ::file a_pmid_2514_2146_260.txt
# ::snt When cells in several wells were confluent, viable cells were quantified by MTS assay and cell numbers were determined by comparing with a standard curve of known cell numbers.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Fri Feb 12, 2016
(a2 / and
:op1 (q / quantify-01
:ARG0 (a / assay-01
:instrument (s3 / small-molecule
:name (n / name :op1 "MTS")
:xref (x / xref :value "PUBCHEM:65521" :prob "10.885861")))
:ARG1 (c / cell
:mod (v / viable)))
:op2 (d / determine-01
:ARG1 (n2 / number
:quant-of (c2 / cell))
:instrument (c3 / compare-01
:ARG1 n2
:ARG2 (c4 / curve
:ARG1-of (s / standard-02)
:quant-of (n3 / number
:mod (c5 / cell)
:ARG1-of (k / know-01)))))
:time (c6 / confluent
:domain (c7 / cell
:location (w / well
:quant (s2 / several)))))
# ::id a_pmid_2514_2146.261
# ::date 2015-06-14T08:48:36
# ::file a_pmid_2514_2146_261.txt
# ::snt B.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Fri Oct 23, 2015
(h / have-li-91 :ARG2 "B")
# ::id a_pmid_2514_2146.262
# ::date 2015-06-14T08:49:00
# ::file a_pmid_2514_2146_262.txt
# ::snt Microscopic imaging of treated M238 cells photographed at 14 days (top) or 44 days (bottom) of treatment.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Mon Dec 21, 2015
(i / image-101
:ARG1 (a3 / and
:op1 (c / cell-line
:name (n / name :op1 "M238")
:ARG1-of (t3 / treat-04)
:ARG1-of (p / photograph-01
:time (a / after
:op1 (t7 / treat-04)
:quant (t4 / temporal-quantity
:quant "14"
:unit (d3 / day))))
:location (t5 / top))
:op2 (c2 / cell-line
:name (n2 / name :op1 "M238")
:ARG1-of t3
:ARG1-of (p2 / photograph-01
:time (a2 / after
:op1 (t / treat-04)
:quant (t6 / temporal-quantity
:quant "44"
:unit (d4 / day))))
:location (b / bottom)))
:mod (m / microscopic))
# ::id a_pmid_2514_2146.263
# ::date 2015-06-14T08:57:44
# ::file a_pmid_2514_2146_263.txt
# ::snt C.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Fri Oct 23, 2015
(h / have-li-91 :ARG2 "C")
# ::id a_pmid_2514_2146.264
# ::date 2015-06-14T08:58:08
# ::file a_pmid_2514_2146_264.txt
# ::snt Quantitative analysis of viable cells for M792 at 44 days (V) or 84 days (E and V + E).
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Mon Jan 4, 2016
(a / analyze-01
:ARG1 (c / cell
:mod (v / viable))
:mod (q / quantitative)
:purpose (a5 / and
:op1 (c2 / cell-line
:name (n / name :op1 "M792")
:ARG1-of (e / expose-01
:ARG2 (s2 / small-molecule
:name (n2 / name :op1 "Vemurafenib")
:xref (x / xref :value "PUBCHEM:42611257" :prob "17.762056"))
:duration (t3 / temporal-quantity
:quant "44"
:unit (d3 / day))))
:op1 (c4 / cell-line
:name (n4 / name :op1 "M792")
:ARG1-of (e3 / expose-01
:ARG2 (a3 / and
:op1 (s / small-molecule
:name (n3 / name :op1 "SCH722984"))
:op2 (c3 / combine-01
:ARG1 s2
:ARG2 s))
:duration (t4 / temporal-quantity
:quant "84"
:unit (d4 / day))))))
# ::id a_pmid_2514_2146.265
# ::date 2015-06-14T09:06:59
# ::file a_pmid_2514_2146_265.txt
# ::snt D.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Fri Oct 23, 2015
(h / have-li-91 :ARG2 "D")
# ::id a_pmid_2514_2146.266
# ::date 2015-06-14T09:07:22
# ::file a_pmid_2514_2146_266.txt
# ::snt Quantitative analysis of viable cells for M238 at 44 days (V), 140 days (E) or 280 days (V + E).
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Mon Jan 4, 2016
(a3 / analyze-01
:ARG1 (o2 / or
:op1 (c / cell
:mod (v / viable)))
:mod (q / quantitative)
:purpose (a / and
:op1 (c2 / cell-line
:name (n / name :op1 "M238")
:ARG1-of (e / expose-01
:ARG2 (s2 / small-molecule
:name (n2 / name :op1 "Vemurafenib")
:xref (x / xref :value "PUBCHEM:42611257" :prob "17.762056"))
:duration (t4 / temporal-quantity
:quant "44"
:unit (d4 / day))))
:op2 (c3 / cell-line
:name (n6 / name :op1 "M238")
:ARG1-of (e3 / expose-01
:ARG2 (s / small-molecule
:name (n3 / name :op1 "SCH722984"))
:duration (t5 / temporal-quantity
:quant "140"
:unit (d5 / day))))
:op3 (c6 / cell-line
:name (n7 / name :op1 "M238")
:ARG1-of (e4 / expose-01
:ARG2 (a6 / and
:op1 s2
:op2 s)
:duration (t6 / temporal-quantity
:quant "280"
:unit (d6 / day))))))
# ::id a_pmid_2514_2146.267
# ::date 2015-06-14T09:19:57
# ::file a_pmid_2514_2146_267.txt
# ::snt The percentage of wells with 1–1,000 (white), 2,000-3,999 (tan), 4,000-5,999 (yellow), 6,000-7,999 (orange), 8,000-9,999 (red) and >10,000 (brown) viable cells are shown.
# ::preferred
# ::annotator SDL-AMR-09
# ::save-date Sun Dec 20, 2015
(s / show-01
:ARG1 (p2 / percentage
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:ARG0-of (h / have-03
:ARG1 (a / and
:op1 (c / cell
:mod (v / viable)
:quant (v2 / value-interval :op1 "1" :op2 "1000")
:ARG1-of (c2 / color-01
:ARG2 (w2 / white-03)))
:op2 (c3 / cell
:mod v
:quant (v3 / value-interval :op1 "2000" :op2 "3999")
:ARG1-of (c4 / color-01
:ARG2 (t / tan)))
:op3 (c5 / cell
:mod v
:quant (v4 / value-interval :op1 "4000" :op2 "5999")
:ARG1-of (c6 / color-01
:ARG2 (y / yellow-02)))
:op4 (c7 / cell
:mod v
:quant (v5 / value-interval :op1 "6000" :op2 "7999")
:ARG1-of (c8 / color-01
:ARG2 (o / orange)))
:op5 (c9 / cell
:mod v
:quant (v6 / value-interval :op1 "8000" :op2 "9999")
:ARG1-of (c10 / color-01
:ARG2 (r / red-02)))
:op6 (c11 / cell
:mod v
:quant (m / more-than :op1 "10000")
:ARG1-of (c12 / color-01
:ARG2 (b4 / brown))))))))
# ::id a_pmid_2514_2146.268
# ::date 2015-06-14T09:32:03
# ::file a_pmid_2514_2146_268.txt
# ::snt Data are representative of triplicate independent experiments.