Annotated spectra of F_02_23_E1_MSA.msf

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Peptides

Confidence Sequence Activation Type Modifications IonScore ΔScore Rank Search Engine Charge Precursor m/z [Da] ΔM [ppm] First Scan Last Scan Annotated Spectrum Peak List
High LAsIDSFDSR CID S3(Phosp) 22.89 1.00 1 Mascot (2) 2 595.75848 0.61 1660 1660 Image Peak List
High SLsGISLNEPK CID S3(Phosp) 25.77 0.35 1 Mascot (2) 2 612.79749 0.39 1639 1639 Image Peak List
High KITtQPINIR CID T4(Phosp) 37.03 0.16 1 Mascot (2) 2 632.34668 3.14 916 916 Image Peak List
High SLGsFRsAANV CID S4(Phosp); S7(Phosp) 27.19 0.37 1 Mascot (2) 2 634.75739 0.18 2059 2059 Image Peak List
High NcKsLENMVK CID C2(Methylthio); S4(Phosp) 37.89 1.00 1 Mascot (2) 2 646.26654 1.01 1495 1495 Image Peak List
High ENsGFGFLLTR CID S3(Phosp) 28.97 1.00 1 Mascot (2) 2 660.80298 0.17 3109 3109 Image Peak List
Medium TPAFLSSsLSKK CID S8(Phosp) 28.26 0.11 1 Mascot (2) 2 673.34406 3.32 1212 1212 Image Peak List
High VMsESEMVSGAR CID S3(Phosp) 49.42 0.39 1 Mascot (2) 2 681.77509 -0.09 1224 1224 Image Peak List
High ALGsFGSFGsFR CID S4(Phosp); S10(Phosp) 53.91 0.20 1 Mascot (2) 2 696.77356 0.91 3483 3483 Image Peak List
High sKSFGNLMEAASK CID S1(Phosp) 40.12 1.00 1 Mascot (2) 2 725.32684 1.36 1385 1385 Image Peak List
High VDsEGVLcGANFK CID S3(Phosp); C8(Methylthio) 44.10 1.00 1 Mascot (2) 2 732.80011 2.04 2454 2454 Image Peak List
High VPsPcLtPIGSGR CID S3(Phosp); C5(Methylthio); T7(Phosp) 20.83 1.00 1 Mascot (2) 2 745.30304 0.48 2337 2337 Image Peak List
High cVsLNNLSDYEK CID C1(Methylthio); S3(Phosp) 30.68 1.00 1 Mascot (2) 2 755.80286 2.01 2248 2248 Image Peak List
High RGsEANWALANSR CID S3(Phosp) 42.14 1.00 1 Mascot (2) 2 756.34424 4.00 972 972 Image Peak List
High DKFGVPAVSLPsPK CID S12(Phosp) 25.68 0.31 1 Mascot (2) 2 761.39020 1.16 2045 2045 Image Peak List
High EyAQITALPRGSR CID Y2(Phospho) 28.13 0.43 1 Mascot (2) 3 514.58722 -0.09 1091 1091 Image Peak List
Low RVSsFEALQPATR CID S4(Phosp) 16.16 2 Mascot (2) 3 514.58722 -0.10 1091 1091 Image Peak List
Medium RYsPPYYsPPR CID S3(Phosp); S8(Phosp) 17.20 1.00 1 Mascot (2) 2 771.81360 1.35 1292 1292 Image Peak List
High AQTGVMtAETGTYR CID T7(Phosp) 26.30 0.31 1 Mascot (2) 2 783.33783 1.13 1079 1079 Image Peak List
High sKSWGITDPELQR CID S1(Phosp) 26.69 0.04 1 Mascot (2) 2 798.87732 3.46 1483 1483 Image Peak List
High SFPGGSGPIRVPscK CID S13(Phosp); C14(Methylthio) 28.06 1.00 1 Mascot (2) 2 807.86438 2.84 2062 2062 Image Peak List
High KDsFSDLLLHLPR CID S3(Phosp) 42.38 0.10 1 Mascot (2) 3 540.94366 1.13 2654 2654 Image Peak List
High LKPFGcNLLYHDR CID C6(Methylthio) 24.04 1.00 1 Mascot (2) 3 541.27289 3.88 1868 1868 Image Peak List
High NsSPPsPFHPAAYK CID S2(Phosp); S6(Phosp) 17.42 0.06 1 Mascot (2) 2 830.33435 0.58 1324 1324 Image Peak List
Medium sFPGGSGPIRVPscK CID S1(Phosp); S13(Phosp); C14(Methylthio) 24.38 0.21 1 Mascot (2) 2 847.84650 1.47 2577 2577 Image Peak List
High VQsLAESNLSSLPDR CID S3(Phosp) 83.44 0.51 1 Mascot (2) 2 848.40137 0.27 1763 1763 Image Peak List
High TRsFDDSPPAAAELR CID S3(Phosp) 51.33 0.02 1 Mascot (2) 2 856.88892 3.80 1093 1093 Image Peak List
High SKsELEQcVTEESK CID S3(Phosp); C8(Methylthio) 52.66 0.15 1 Mascot (2) 2 861.85205 0.33 1329 1329 Image Peak List
High TPsKISSIFcGTQDK CID S3(Phosp); C10(Methylthio) 35.54 0.11 1 Mascot (2) 2 869.38373 0.79 2192 2192 Image Peak List
High YGAGIGPGVYDIHsPR CID S14(Phosp) 37.28 1.00 1 Mascot (2) 2 869.90228 1.36 1478 1478 Image Peak List
High APMIIHHADsELMGR CID S10(Phosp) 27.02 1.00 1 Mascot (2) 3 586.60028 -0.05 1168 1168 Image Peak List
High LDAPRPtTPKPPsPR CID T7(Phosp); S13(Phosp) 21.11 0.24 1 Mascot (2) 3 597.28510 0.49 622 622 Image Peak List
High VEPEEsESDDVIIVR CID S6(Phosp) 33.45 0.09 1 Mascot (2) 2 898.40411 0.63 1828 1828 Image Peak List
High sASFKEESDFFADLK CID S1(Phosp) 50.09 0.04 1 Mascot (2) 2 900.89093 0.95 2608 2608 Image Peak List
High INSAEsMELWASQQK CID S6(Phosp) 27.66 0.14 1 Mascot (2) 3 601.26611 0.59 2127 2127 Image Peak List
High VLsPYSLPASLLHSER CID S3(Phosp) 44.90 0.49 1 Mascot (2) 2 924.96753 1.18 2801 2801 Image Peak List
High GFDASLsEDDIKNTLR CID S7(Phosp) 28.00 0.21 1 Mascot (2) 2 930.92358 1.23 1947 1947 Image Peak List
High SMIPQNLGsFKEESSK CID S9(Phosp) 33.54 1.00 1 Mascot (2) 2 931.42554 1.91 1758 1758 Image Peak List
High FSYNSYPDsAESsPR CID S9(Phosp); S13(Phosp) 28.91 0.24 1 Mascot (2) 2 933.83551 0.95 1562 1562 Image Peak List
High VFcSQVsNLsTEMAR CID C3(Methylthio); S7(Phosp); S10(Phosp) 37.82 0.16 1 Mascot (2) 2 939.35858 3.47 2913 2913 Image Peak List
High INsAEsMELWASQQK CID S3(Phosp); S6(Phosp) 39.05 1.00 1 Mascot (2) 2 941.38007 2.02 2540 2540 Image Peak List
High YVEEWVGPGSPMNsPR CID S14(Phosp) 35.40 1.00 1 Mascot (2) 2 942.90045 -2.70 2094 2094 Image Peak List
High TPYHPMMDDDGSLsPR CID S14(Phosp) 43.25 0.26 1 Mascot (2) 2 949.87762 1.59 1180 1180 Image Peak List
High SALPLKtPENsPTATDT CID T7(Phosp); S11(Phosp) 24.65 0.16 1 Mascot (2) 2 951.91229 2.04 1600 1600 Image Peak List
High LIEEVSHSSGsPNPVsD CID S11(Phosp); S16(Phosp) 31.16 0.45 1 Mascot (2) 2 957.38245 0.51 1306 1306 Image Peak List
High VTSESDFMtEyVVTR CID T9(Phosp); Y11(Phospho) 74.37 0.54 1 Mascot (2) 2 962.37994 2.20 2400 2400 Image Peak List
High SKtPEPQPTYFEPSSR CID T3(Phosp) 20.64 0.10 1 Mascot (2) 3 644.29144 0.76 1044 1044 Image Peak List
High VKEGDHIIIGQcRPLSK CID C12(Methylthio) 45.12 1.00 1 Mascot (2) 3 647.01355 0.41 949 949 Image Peak List
High VPEAQITNsATPTTtPR CID S9(Phosp); T15(Phosp) 43.59 0.09 1 Mascot (2) 2 972.43201 2.85 1026 1026 Image Peak List
High AAAAVTTTTTDSLAsDDDR CID S15(Phosp) 67.23 0.28 1 Mascot (2) 2 981.42114 0.70 937 937 Image Peak List
High SIPEGAVVcNVEHHVGDR CID C9(Methylthio) 41.91 1.00 1 Mascot (2) 3 655.30914 0.76 1397 1397 Image Peak List
High YVEEWVGPGsPMNsPR CID S10(Phosp); S14(Phosp) 51.51 1.00 1 Mascot (2) 2 982.88641 0.26 2370 2370 Image Peak List
High AHGTDPsPPMsPILGATR CID S7(Phosp); S11(Phosp) 48.12 0.31 1 Mascot (2) 2 982.92169 1.17 1796 1796 Image Peak List
High TKSETDFMtEyVVTR CID T9(Phosp); Y11(Phospho) 58.35 0.24 1 Mascot (2) 2 983.89990 0.99 1903 1903 Image Peak List
High TPYHPMMDDDGsLsPR CID S12(Phosp); S14(Phosp) 32.03 1.00 1 Mascot (2) 3 660.24249 0.82 1450 1450 Image Peak List
High EDDKVALQEDNcADDVR CID C12(Methylthio) 26.86 1.00 1 Mascot (2) 3 660.94757 0.36 1133 1133 Image Peak List
Low SEEQEEGsYRKPSYGR CID S8(Phosp) 24.25 1.00 1 Mascot (2) 3 661.28119 0.11 163 163 Image Peak List
High SGFIHHcLVSDLEHDTK CID C7(Methylthio) 37.69 1.00 1 Mascot (2) 2 992.45544 -0.81 1373 1373 Image Peak List
High tNSSPGNFESPLDRPMR CID T1(Phosp) 25.65 0.04 1 Mascot (2) 2 992.93347 0.66 1471 1471 Image Peak List
High TLEAPLPGSLsAVNNATTR CID S11(Phosp) 32.16 0.06 1 Mascot (2) 2 996.49310 -0.46 2388 2388 Image Peak List
High NMQQLNQsPDIDGELSK CID S8(Phosp) 43.46 1.00 1 Mascot (2) 2 998.93829 0.53 1632 1632 Image Peak List
Medium KLDTSLsINLRPPPIDR CID S7(Phosp) 27.24 0.26 1 Mascot (2) 3 672.36255 2.22 2001 2001 Image Peak List
High TIEKEIsDDEEEEEKK CID S7(Phosp) 34.79 0.37 1 Mascot (2) 3 677.62823 0.55 631 631 Image Peak List
High KPVYNLDDsDDDDFVPK CID S9(Phosp) 44.41 1.00 1 Mascot (2) 2 1031.43408 -3.92 1842 1842 Image Peak List
High AVGsHcSLSEMDDLDLTR CID S4(Phosp); C6(Methylthio) 73.49 0.32 1 Mascot (2) 2 1037.91809 0.41 2545 2545 Image Peak List
High FGIVEGLMTTVHSITAtQK CID T17(Phosp) 47.58 0.04 1 Mascot (2) 3 705.01837 0.26 3417 3417 Image Peak List
High SEINDEDEAsGEEELLEK CID S10(Phosp) 88.63 0.64 1 Mascot (2) 2 1058.43152 3.18 1749 1749 Image Peak List
High LKEHMQYcMDHPDEISK CID C8(Methylthio) 34.08 1.00 1 Mascot (2) 3 717.31024 -0.60 767 767 Image Peak List
High SNRYsDEDEEEEEVAGGR CID S5(Phosp) 68.10 0.29 1 Mascot (2) 2 1075.91626 4.90 680 680 Image Peak List
High ADADDDKLVDGLNWAcGQGR CID C16(Methylthio) 33.96 1.00 1 Mascot (2) 3 722.31854 1.07 2710 2710 Image Peak List
High KKEEsEEEEGDFGFDLFG CID S5(Phosp) 51.02 1.00 1 Mascot (2) 2 1086.44006 1.60 3067 3067 Image Peak List
High VSGsEHGFSLNEPLGISENK CID S4(Phosp) 38.98 0.31 1 Mascot (2) 2 1090.99841 1.60 2003 2003 Image Peak List
High TSsFRDPLSVSDSASPIPSR CID S3(Phosp) 33.01 0.09 1 Mascot (2) 2 1093.51282 2.63 1957 1957 Image Peak List
High KLEDIVPSSHNcDVPHVNR CID C12(Methylthio) 46.08 1.00 1 Mascot (2) 2 1103.03308 2.21 1205 1205 Image Peak List
High LKTESSsADNIQEDDGSSLR CID S7(Phosp) 54.29 0.15 1 Mascot (2) 2 1116.48950 2.36 673 673 Image Peak List
High VLSHAVSEsDVsVHSTFASR CID S9(Phosp); S12(Phosp) 32.85 0.27 1 Mascot (2) 3 758.99738 -0.74 1331 1331 Image Peak List
High VSFDEESPPKLsPEAKPLTK CID S12(Phosp) 30.35 0.33 1 Mascot (2) 3 760.37714 -1.72 1364 1364 Image Peak List
High ANLsPAGDKANVEEPMVYATK CID S4(Phosp) 40.56 1.00 1 Mascot (2) 3 762.35614 0.79 1448 1448 Image Peak List
Medium EKPLVQVLsDDDsEVKEAK CID S9(Phosp); S13(Phosp) 27.69 1.00 1 Mascot (2) 2 1145.02063 1.74 1511 1511 Image Peak List
High HYSEDVGEVQASQEKPVsPK CID S18(Phosp) 30.67 1.00 1 Mascot (2) 3 765.34918 -0.32 512 512 Image Peak List
High QNLRDGEVsDVEEDEYEAK CID S9(Phosp) 41.45 1.00 1 Mascot (2) 2 1152.98157 2.34 1063 1063 Image Peak List
High cDGGDDIDDVQSHSTDEPVKK CID C1(Methylthio) 28.74 1.00 1 Mascot (2) 3 769.32513 2.61 508 508 Image Peak List
High IGIWQYGDIEsDDEDTGPAR CID S11(Phosp) 39.79 0.45 1 Mascot (2) 3 772.99475 1.71 2815 2815 Image Peak List
High EANVDGDTAsQHEAAWLPGKR CID S10(Phosp) 34.71 0.03 1 Mascot (2) 3 778.01825 2.02 1028 1028 Image Peak List
High WDPDsDMDVDDDRKPIPSR CID S5(Phosp) 36.24 1.00 1 Mascot (2) 3 780.32715 0.93 1341 1341 Image Peak List
High tPPTNASLDYPSADSEHVSKR CID T1(Phosp) 30.07 0.20 1 Mascot (2) 3 784.68793 -0.52 657 657 Image Peak List
High SHsVSGDLHGVQPDPIAADILR CID S3(Phosp) 60.28 0.15 1 Mascot (2) 3 788.71722 1.12 2055 2055 Image Peak List
High ATGAFILtASHNPGGPTEDFGIK CID T8(Phosp) 30.88 0.03 1 Mascot (2) 3 794.37500 -2.93 2566 2566 Image Peak List
High STPGSPAHPPGARsPPPSYLSNK CID S14(Phosp) 16.45 1.00 1 Mascot (2) 3 794.71356 0.94 543 543 Image Peak List
High KFsEQNIGAPPSYEEAVSDSR CID S3(Phosp) 65.18 0.75 1 Mascot (2) 2 1196.03125 2.13 1593 1593 Image Peak List
High SSIASVPITDTTHVKSEtGsPR CID T18(Phosp); S20(Phosp) 17.92 1.00 1 Mascot (2) 3 810.70325 0.06 1189 1189 Image Peak List
High ETLESALLKsPsPDNNNVSEK CID S10(Phosp); S12(Phosp) 30.85 0.48 1 Mascot (2) 3 811.36023 1.26 1996 1996 Image Peak List
High SsDSLSGTNELLNINSETPMKK CID S2(Phosp) 23.93 0.13 1 Mascot (2) 3 815.71478 3.36 2073 2073 Image Peak List
High GYDGADsPIREsPSRsPPAEE CID S7(Phosp); S12(Phosp); S16(Phosp) 28.86 0.21 1 Mascot (2) 3 819.64032 0.76 1117 1117 Image Peak List
High VVEEKPAsPEPVKAEAEKPVEK CID S8(Phosp) 23.02 1.00 1 Mascot (2) 3 824.08783 0.46 278 278 Image Peak List
High VQEVPHEGPMcDLLWSDPDDR CID C11(Methylthio) 24.55 1.00 1 Mascot (2) 3 828.69550 3.61 2862 2862 Image Peak List
High sAPTtPINQNAAAAFAAVSEEER CID S1(Phosp); T5(Phosp) 68.36 0.07 1 Mascot (2) 2 1253.04150 2.46 3168 3168 Image Peak List
Low EVASVYGIAFEAKysYSSAFR CID Y14(Phospho); S15(Phosp) 15.89 5 Mascot (2) 2 1253.04150 -0.74 3168 3168 Image Peak List
High AEALAALTSAFNSSPSsKsPPRR CID S17(Phosp); S19(Phosp) 29.89 0.17 1 Mascot (2) 3 835.72498 2.71 2190 2190 Image Peak List
High KQEEAPsTPQRPLSPLGEAcSR CID S7(Phosp); C20(Methylthio) 19.45 1.00 1 Mascot (2) 3 836.38538 -0.15 1348 1348 Image Peak List
High HSVLHDQETGEEYcPLDHLIK CID C14(Methylthio) 44.84 1.00 1 Mascot (2) 3 837.05475 0.39 1971 1971 Image Peak List
High SFDRESPsNLDSTSGISQPHNR CID S8(Phosp) 60.10 0.28 1 Mascot (2) 3 837.69995 -1.75 750 750 Image Peak List
High HIEDTWMQELNVNRPPtPTR CID T17(Phosp) 22.69 0.04 1 Mascot (2) 3 838.72638 1.97 2064 2064 Image Peak List
High KGYTsDEELEELDSPLTSIVDK CID S5(Phosp) 41.73 1.00 1 Mascot (2) 3 850.05713 -0.88 3011 3011 Image Peak List
High TKNsNHDENEFISFEPNQNTK CID S4(Phosp) 19.69 1.00 1 Mascot (2) 3 858.37036 0.94 1273 1273 Image Peak List
High AHEGEDEGAGLsELEEEDDDAKR CID S12(Phosp) 56.45 1.00 1 Mascot (2) 3 861.01141 -0.55 1170 1170 Image Peak List
High HPQWQSDDGGDNsEPESPSDSLR CID S13(Phosp) 31.11 0.35 1 Mascot (2) 3 874.01202 -0.22 960 960 Image Peak List
High AGDStPEELANATQVQGDYLPIVR CID T5(Phosp) 81.97 0.74 1 Mascot (2) 2 1312.62195 4.78 2920 2920 Image Peak List
High LVPHTVASQSEVDVAsPVsEKAPK CID S16(Phosp); S19(Phosp) 27.68 1.00 1 Mascot (2) 3 879.08606 0.72 1114 1114 Image Peak List
High TSGLPSQTSGAGSAYATLPQLPLsPR CID S24(Phosp) 34.24 0.15 1 Mascot (2) 3 879.77216 3.72 2783 2783 Image Peak List
High VEEKEEsDEEDYGGDFGLFDEE CID S7(Phosp) 57.11 0.44 1 Mascot (2) 2 1323.98865 -2.57 3007 3007 Image Peak List
High TLPVAVVEVVKPEsPVLVIVEKPK CID S14(Phosp) 47.39 1.00 1 Mascot (2) 3 883.84851 2.50 2743 2743 Image Peak List
High RDDsFQKVNDHPISTVESNLSAK CID S4(Phosp) 42.95 0.21 1 Mascot (2) 3 889.75226 0.41 1245 1245 Image Peak List
High VHDMVVADADQDDGtDDDNDLGEK CID T15(Phosp) 57.64 1.00 1 Mascot (2) 3 890.34564 1.11 1152 1152 Image Peak List
High ITsSPKQEIGTGEATEQEEGKEQK CID S3(Phosp) 20.68 0.05 1 Mascot (2) 3 895.41620 1.52 414 414 Image Peak List
High HPQWQSDDGGDNsEPEsPSDSLR CID S13(Phosp); S17(Phosp) 32.77 0.03 1 Mascot (2) 2 1350.49976 1.42 1191 1191 Image Peak List
High KRsAPTTPINQNAAAAFAAVSEEER CID S3(Phosp) 42.97 0.09 1 Mascot (2) 3 903.77173 0.48 1954 1954 Image Peak List
High EILQSEsFKEEGYLASELQEAEK CID S7(Phosp) 50.19 0.26 1 Mascot (2) 3 913.08917 1.34 2699 2699 Image Peak List
High SPTVVTVQPssPRFPISTPTAGAQR CID S10(Phosp); S11(Phosp) 26.96 0.26 1 Mascot (2) 3 914.44250 2.52 2118 2118 Image Peak List
High FPDDKDGEDEKSGDsGGENLMDSDK CID S15(Phosp) 36.47 0.14 1 Mascot (2) 3 923.02539 1.96 876 876 Image Peak List
High NILKPADAVTPSTHSSTFFSVQsPR CID S23(Phosp) 36.20 0.22 1 Mascot (2) 3 923.12018 0.29 1891 1891 Image Peak List
High EIDEHLLAEEEEVKtPETPSLVGK CID T15(Phosp) 19.80 1.00 1 Mascot (2) 3 924.78052 1.51 2006 2006 Image Peak List
High MKLPLDIDsPTQSENSSSSQQtPK CID S9(Phosp); T22(Phosp) 26.10 1.00 1 Mascot (2) 3 926.73383 -2.61 2386 2386 Image Peak List
High VVYVAPPRPPsPVREGsEEGsSPR CID S11(Phosp); S17(Phosp); S21(Phosp) 37.94 0.03 1 Mascot (2) 3 930.40955 1.54 1490 1490 Image Peak List
High KRsAPtTPINQNAAAAFAAVSEEER CID S3(Phosp); T6(Phosp) 61.10 1.00 1 Mascot (2) 3 930.42426 -2.67 2164 2164 Image Peak List
High KPEDGsAVAAAPVVVPPPVEEAHPVEK CID S6(Phosp) 16.29 1.00 1 Mascot (2) 3 933.80414 0.45 1434 1434 Image Peak List
High RANsYHSYSQSPPYDyQYEER CID S4(Phosp); Y16(Phospho) 17.09 0.06 1 Mascot (2) 3 934.02960 1.06 1163 1163 Image Peak List
High ITSNNMDHHScAEEYsDEDVEVR CID C11(Methylthio); S16(Phosp) 48.90 0.53 1 Mascot (2) 3 936.02039 1.37 1005 1005 Image Peak List
High AKETGPEIETAVDGEsVHEIETTER CID S16(Phosp) 41.48 0.41 1 Mascot (2) 3 936.42657 1.13 1334 1334 Image Peak List
High ISPDIAEQHQLAVIDcLEDPDDTLK CID C16(Methylthio) 32.51 1.00 1 Mascot (2) 3 942.11993 2.54 3144 3144 Image Peak List
High TGIWEYGDIQsDDEDNVPVRKPGR CID S11(Phosp) 35.70 0.53 1 Mascot (2) 3 942.76575 3.37 1803 1803 Image Peak List
High IRPAGSREDMtFGAEESKDESGETR CID T11(Phosp) 49.92 0.02 1 Mascot (2) 3 945.74823 1.72 585 585 Image Peak List
High KSPTVVTVQPSsPRFPIsTPTAGAQR CID S12(Phosp); S18(Phosp) 23.77 1.00 1 Mascot (2) 3 957.13702 -1.57 1702 1702 Image Peak List
High HLESFNFDSEIPADLPVTSsSHELK CID S20(Phosp) 22.32 0.09 1 Mascot (2) 3 960.44403 1.36 2491 2491 Image Peak List
High RGSGVcHDDSGPTVNATTIDELHSLQK CID C6(Methylthio) 47.14 1.00 1 Mascot (2) 3 961.78131 -2.33 1446 1446 Image Peak List
High GSDNSVSASSSTAEREsDEDSSNALAVR CID S17(Phosp) 20.62 0.24 1 Mascot (2) 3 970.07532 1.26 762 762 Image Peak List
High ASDNSVSASSSTAEREsDEDSSNALAVR CID S17(Phosp) 46.83 0.17 1 Mascot (2) 3 974.74738 1.43 774 774 Image Peak List
High REDQEPGLQtPDLILsEVEDEKPK CID T10(Phosp); S16(Phosp) 41.51 1.00 1 Mascot (2) 3 975.77917 2.12 2745 2745 Image Peak List
High FEDQsEtSSSHGGITAPGPISVAQLPR CID S5(Phosp); T7(Phosp) 41.93 0.19 1 Mascot (2) 3 976.76990 4.70 2549 2549 Image Peak List
High DWLESQGHQYIVTDDKEGPDcELEK CID C21(Methylthio) 62.83 1.00 1 Mascot (2) 3 994.10504 0.98 2139 2139 Image Peak List
High ISHSTGSETLDQNMVLGEVsPKDEESR CID S20(Phosp) 51.40 0.51 1 Mascot (2) 3 1009.12183 1.71 1439 1439 Image Peak List
High IELQSQSWPMQQSFsPEKENGQTGAR CID S15(Phosp) 51.99 0.27 1 Mascot (2) 3 1015.12378 -1.61 2087 2087 Image Peak List
High LsKSDDSLGSQFLMSHPQASTGQQEPAK CID S2(Phosp) 42.46 0.02 1 Mascot (2) 3 1018.80188 1.16 1518 1518 Image Peak List
High TTPASASASyFHTMSsDDEDEKEVHR CID Y10(Phospho); S16(Phosp) 29.03 0.10 1 Mascot (2) 3 1020.06531 0.11 1184 1184 Image Peak List
High EKPSSPPtFDDGPtSPPASLHEPEPSAK CID T8(Phosp); T14(Phosp) 21.52 1.00 1 Mascot (2) 3 1021.43561 -4.78 1425 1425 Image Peak List
High SSWTSESYQLKPQSSFSGSHPSGsPNAR CID S24(Phosp) 34.28 0.03 1 Mascot (2) 3 1026.12317 3.77 1271 1271 Image Peak List
High WDsTTSEEAKENLIFQSGGDREEVDR CID S3(Phosp) 33.26 0.03 1 Mascot (2) 3 1026.78687 4.38 1735 1735 Image Peak List
High FSSLEKPQFDHQNSsGSNYDDLTPHR CID S15(Phosp) 23.08 0.09 1 Mascot (2) 3 1029.45154 4.01 1233 1233 Image Peak List
High ASLsTGNIPEMFDGRPSFTEGSGLEASPR CID S4(Phosp) 60.08 0.07 1 Mascot (2) 3 1030.80054 -0.17 2778 2778 Image Peak List
High GMVSSGGPVsPGPVYPGGRPGAGGLMPGMPGTR CID S10(Phosp) 48.51 0.47 1 Mascot (2) 3 1039.82373 3.09 2283 2283 Image Peak List
High NVEGsNEDEEEEIEEDGEEISEVDHAK CID S5(Phosp) 66.39 1.00 1 Mascot (2) 3 1047.41138 -0.92 2206 2206 Image Peak List
High FHSMEKPQFDHQNsSGSNYDDLTPQR CID S14(Phosp) 35.20 0.14 1 Mascot (2) 3 1049.11121 2.67 993 993 Image Peak List
High HDAIASGSPLPVQASGSPLSVQASKPADSsPK CID S30(Phosp) 27.55 0.04 1 Mascot (2) 3 1056.18726 1.72 1537 1537 Image Peak List
High SSIQDDEsNAEETDDQHLASDHATDITR CID S8(Phosp) 39.78 0.18 1 Mascot (2) 3 1060.77161 4.04 930 930 Image Peak List
High sTSSNPADDIPLTEAKDEEEASEVAGLEAK CID S1(Phosp) 58.44 0.07 1 Mascot (2) 3 1061.81018 1.81 2482 2482 Image Peak List
High KFSEQNIGAPPSYEEAVSDSRsPVYSER CID S22(Phosp) 31.25 0.03 1 Mascot (2) 3 1070.49084 4.85 1569 1569 Image Peak List
High GNWGTTEDDIPPTSEEPTTEVEKsPVAEK CID S24(Phosp) 24.27 0.17 1 Mascot (2) 3 1075.14600 0.95 1574 1574 Image Peak List
High RKGDGDDEEsEDESAENVVNVEDFLVQK CID S10(Phosp) 79.40 0.37 1 Mascot (2) 3 1078.13306 1.33 2596 2596 Image Peak List
High IEEEsENEEEEEGEEEDDDEEVKEKK CID S5(Phosp) 54.43 1.00 1 Mascot (2) 3 1078.75195 1.07 398 398 Image Peak List
High ASEAEHEDPVDDIKsNDDRDFPTEQAPK CID S15(Phosp) 44.88 0.49 1 Mascot (2) 3 1079.13342 4.83 1096 1096 Image Peak List
High StSsNPADDIPLTEAKDEEEASEVAGLEAK CID T2(Phosp); S4(Phosp) 57.00 0.05 1 Mascot (2) 3 1088.46692 2.96 2878 2878 Image Peak List
High HSSSIsPALsPQPLGSYGSYGQNIPMGSQR CID S6(Phosp); S10(Phosp) 15.98 1.00 1 Mascot (2) 3 1088.48157 2.21 2451 2451 Image Peak List
High IISQLEPEVLsPIKPADDQLSLSDLDMVK CID S11(Phosp) 83.89 0.45 1 Mascot (2) 3 1091.89343 2.45 3471 3471 Image Peak List
High KTDGStTPAYAHGQHHSIFSPATGAVSDSSLK CID T6(Phosp) 43.13 0.07 1 Mascot (2) 3 1112.51575 -0.08 979 979 Image Peak List
High HDSSSSYKsDADDDVDFSETTSNPTWLEK CID S9(Phosp) 55.92 0.16 1 Mascot (2) 3 1115.12073 1.22 1915 1915 Image Peak List
High YQSTYEGYGsPIREEPPPPYSYSEPQSR CID S10(Phosp) 36.79 0.24 1 Mascot (2) 3 1115.48975 3.78 1784 1784 Image Peak List
High DAPAEsDAEDEPEDDEGGDDSDSESKAEETK CID S6(Phosp) 47.94 1.00 1 Mascot (2) 3 1117.07385 1.11 484 484 Image Peak List
High HQHSQSMDGsMNINEMLMSGNEDDSAIDAK CID S10(Phosp) 47.01 0.15 1 Mascot (2) 3 1124.77893 -1.55 2125 2125 Image Peak List
High KEEATPAPAVVEtPVKEPETTTTAPVAEPPKP CID T13(Phosp) 48.20 0.29 1 Mascot (2) 3 1131.23413 -1.29 1285 1285 Image Peak List
High YFFPsDTEDQGDDSKTQEESDAETPtGLK CID S5(Phosp); T26(Phosp) 36.89 0.41 1 Mascot (2) 3 1133.11426 2.60 2332 2332 Image Peak List
High STSssPPPPPPSSSLPQQEQEQDQQQLPLR CID S4(Phosp); S5(Phosp) 35.34 0.14 1 Mascot (2) 3 1144.17944 1.07 2048 2048 Image Peak List
High TEEDENDDEDHEHKDDKtSPDSIVMVEAK CID T18(Phosp) 47.28 0.11 1 Mascot (2) 3 1146.46790 1.26 694 694 Image Peak List
High EFVLINEDESEyDTEMsGDDEVAKPLVSK CID Y12(Phospho); S17(Phosp) 33.72 0.29 1 Mascot (2) 3 1150.14685 -1.63 3004 3004 Image Peak List
High sPEHALFTKPVYDQTEQLPPAPWETQEPR CID S1(Phosp) 39.46 0.46 1 Mascot (2) 3 1157.88220 1.80 2512 2512 Image Peak List
High EIsHDSsDEEDGDGGKNTNEMLNYGLTIASK CID S3(Phosp); S7(Phosp) 44.56 0.04 1 Mascot (2) 3 1162.80554 2.62 2479 2479 Image Peak List
High VLYDVLTHEIVDEDSSsGGEETGSNNAEARPR CID S17(Phosp) 69.81 0.11 1 Mascot (2) 3 1176.19128 -0.40 2136 2136 Image Peak List
High MLQSGMPLDDRPEGQRsPSPEPVyDNMGIR CID S17(Phosp); Y24(Phospho) 34.64 0.26 1 Mascot (2) 3 1178.17444 -0.29 2246 2246 Image Peak List
High EATADMSEEFsEGEKGDIISDISTHGESTKPR CID S11(Phosp) 49.47 0.20 1 Mascot (2) 3 1178.51550 1.59 1896 1896 Image Peak List
High DAPAEsDAEEEAEDDDNEGDDSDNESKSEETK CID S6(Phosp) 85.12 0.59 1 Mascot (2) 3 1184.75830 0.80 634 634 Image Peak List
High FNNHsPEENNNSPLSGSSATNTNETELSLMLK CID S5(Phosp) 47.20 0.21 1 Mascot (2) 3 1186.19507 4.82 2421 2421 Image Peak List
High EATADMsEEFsEGEKGDIISDISTHGESTKPR CID S7(Phosp); S11(Phosp) 49.09 0.47 1 Mascot (2) 3 1205.17126 1.82 2104 2104 Image Peak List
High YEEMNKPSAPLTSHEPAMIPVAEEPDDsPIHGR CID S28(Phosp) 34.63 1.00 1 Mascot (2) 3 1242.22217 -3.79 1630 1630 Image Peak List
High EVEDVVGESESGEVYTLssGDEAETTSRDDVVDK CID S18(Phosp); S19(Phosp) 33.71 0.32 1 Mascot (2) 3 1264.85400 1.39 2171 2171 Image Peak List
High NLEEGsDEsEKsRSEINDEDEAsGEEELLEK CID S6(Phosp); S9(Phosp); S12(Phosp); S23(Phosp) 18.98 1.00 1 Mascot (2) 3 1272.80420 3.15 2262 2262 Image Peak List
High SSAVQEEQFGEHTEPcSSEIKDESHGKEESVEVK CID C16(Methylthio) 38.49 1.00 1 Mascot (2) 3 1274.56030 -2.38 1072 1072 Image Peak List
High SKDNLYEQKPEEPVPVIPAAsPTNDTSAAGSSFASR CID S21(Phosp) 44.38 0.05 1 Mascot (2) 3 1280.94006 2.37 1824 1824 Image Peak List
High IDLDKPEVEDDDDNDEDDsEDDDEAEGHDGEAGGR CID S19(Phosp) 90.50 1.00 1 Mascot (2) 3 1304.80688 -1.54 1023 1023 Image Peak List
High DAETVNQTSHPTEEEAQVTVSSNADVEDSHETVsPR CID S34(Phosp) 78.10 0.10 1 Mascot (2) 3 1325.90588 0.75 1124 1124 Image Peak List
High AEDEEDAsDFEPEENGVEEDIDEGEDDENDNSGGAGK CID S8(Phosp) 66.62 1.00 1 Mascot (2) 3 1346.49365 4.41 2311 2311 Image Peak List
High FAAPSSLsEDDDDDDDDDPDYVEEEEEPLVSHRPR CID S8(Phosp) 123.17 0.15 1 Mascot (2) 3 1362.54468 -0.27 2050 2050 Image Peak List

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Search Summary

Workflow created with Discoverer version: 1.3.0.339 (DBVersion:78)

================================================================================

Search name: F_02_23_E1_MSA
Search description: -
Search date: 09/13/2013 10:11:40

================================================================================

The pipeline tree:
------------------

    |-(0) Spectrum Files
        |-(1) Spectrum Selector
            |-(2) Mascot
                |-(3) Percolator

================================================================================

Search name: F_02_23_E1_MSA
Search description: -
Search date: 09/13/2013 10:11:40

================================================================================

The pipeline tree:
------------------

    |-(0) Spectrum Files
        |-(1) Spectrum Selector
            |-(2) Mascot
                |-(3) Percolator

------------------------------------------------------------------------------
Processing node 0: Spectrum Files
------------------------------------------------------------------------------

Input Data:
-----------------------------
File Name(s): F:\Cytosol\M15F15 022012\Raw files\F_02_23_E1.raw

------------------------------------------------------------------------------
Processing node 1: Spectrum Selector
------------------------------------------------------------------------------

1. General Settings:
-----------------------------
Precursor Selection: Use MS1 Precursor
Use New Precursor Reevaluation: True

2. Spectrum Properties Filter:
-----------------------------
Lower RT Limit: 0
Upper RT Limit: 0
First Scan: 0
Last Scan: 0
Lowest Charge State: 0
Highest Charge State: 0
Min. Precursor Mass: 400 Da
Max. Precursor Mass: 10000 Da
Total Intensity Threshold: 0
Minimum Peak Count: 1

3. Scan Event Filters:
-----------------------------
MS Order: Is MS2
Activation Type: Is CID
Scan Type: Is Full

4. Peak Filters:
-----------------------------
S/N Threshold (FT-only): 3

5. Replacements for Unrecognized Properties:
-----------------------------
Unrecognized Charge Replacements: Automatic
Unrecognized Mass Analyzer Replacements: ITMS
Unrecognized MS Order Replacements: MS2
Unrecognized Activation Type Replacements: CID
Unrecognized Polarity Replacements: +

6. Just for Testing:
-----------------------------
Precursor Clipping Range Before: 2.5 Da
Precursor Clipping Range After: 5.5 Da

------------------------------------------------------------------------------
Processing node 2: Mascot
------------------------------------------------------------------------------

1. Input Data:
-----------------------------
Protein Database: TAIR10
Enzyme Name: Trypsin
Maximum Missed Cleavage Sites: 2
Instrument: ESI-TRAP
Taxonomy: . . . . . . Arabidopsis thaliana (thale cress)

1.1 Peptide Scoring Options:
-----------------------------
Peptide Cut Off Score: 10
Peptide Without Protein Cut Off Score: 5

1.2 Protein Scoring Options:
-----------------------------
Use MudPIT Scoring: Automatic
Protein Relevance Threshold: 20
Protein Relevance Factor: 1

2. Tolerances:
-----------------------------
Precursor Mass Tolerance: 5 ppm
Fragment Mass Tolerance: 0.6 Da
Use Average Precursor Mass: False

3. Dynamic Modifications:
-----------------------------
1. Dynamic Modification: Phosphogood (ST)
2. Dynamic Modification: Phospho (Y)

4. Static Modifications:
-----------------------------
1. Static Modification: Methylthio (C)

------------------------------------------------------------------------------
Processing node 3: Percolator
------------------------------------------------------------------------------

1. Input Data:
-----------------------------
Maximum Delta Cn: 0.05

2. Decoy Database Search:
-----------------------------
Target FDR (Strict): 0.01
Target FDR (Relaxed): 0.05
Validation based on: q-Value

================================================================================

Processing details:

09/13/2013 10:15 AM (2):Mascot: Total search time was 2 min 24 s.
09/13/2013 10:15 AM (3):Percolator: Performing percolator for Mascot (2) took 33.1 s.
09/13/2013 10:15 AM (2):Mascot: Search completed
09/13/2013 10:15 AM (2):Mascot: Protein grouping (204 groups) took: 0.29 s
09/13/2013 10:15 AM (2):Mascot: 318 protein(s) + 2 decoy proteins scored and inserted into result file in 0.96 s
09/13/2013 10:15 AM (2):Mascot: 318 protein(s) scored
09/13/2013 10:15 AM (2):Mascot: Result finalization started
09/13/2013 10:15 AM (3):Percolator: Start reading Percolator results
09/13/2013 10:15 AM (3):Percolator: Merging results from 3 datasets
09/13/2013 10:15 AM (3):Percolator: Found 477 target PSMs scoring over 1% FDR level on testset
09/13/2013 10:15 AM (3):Percolator: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
09/13/2013 10:15 AM (3):Percolator: 5.99 -0.177 1.98 4.16 -1.49 -0.0394 1.16 -0.226 -1.98 3.09 0 -0.39 0.39 0 0 0 -0.847 0 -2.88 0.435 2.62 3.46 0.703 1.75 0.608 4.96 0.442 -2.2 0.593 -2.2 0.326 1.43 -5.24
09/13/2013 10:15 AM (3):Percolator: IonScore Delta Cn From Second PSM Binomial Score Isolation Interference [%] MH+ [Da] Delta Mass [Da] Delta Mass [ppm] Absolute Delta Mass [Da] Absolute Delta Mass [ppm] Peptide Length Is z=1 Is z=2 Is z=3 Is z=4 Is z=5 Is z>5 # Missed Cleavages Log Peptides Matched Log Total Intensity Fraction Matched Intensity [%] Fragment Coverage Series A, B, C [%] Fragment Coverage Series X, Y, Z [%] Log Matched Fragment Series Intensities A, B, C Log Matched Fragment Series Intensities X, Y, Z Longest Sequence Series A, B, C Longest Sequence Series X, Y, Z IQR Fragment Delta Mass [Da] IQR Fragment Delta Mass [ppm] Mean Fragment Delta Mass [Da] Mean Fragment Delta Mass [ppm] Mean Absolute Fragment Delta Mass [Da] Mean Absolute Fragment Delta Mass [ppm] m0
09/13/2013 10:15 AM (3):Percolator: # first line contains normalized weights, second line the raw weights
09/13/2013 10:15 AM (3):Percolator: Obtained weights (only showing weights of first cross validation set)
09/13/2013 10:15 AM (3):Percolator: Iteration 10 : After the iteration step, 569 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 9 : After the iteration step, 569 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 8 : After the iteration step, 569 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 7 : After the iteration step, 569 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 6 : After the iteration step, 569 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 5 : After the iteration step, 569 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 4 : After the iteration step, 568 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 3 : After the iteration step, 565 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 2 : After the iteration step, 559 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: Iteration 1 : After the iteration step, 540 target PSMs with q<0.01 were estimated by cross validation
09/13/2013 10:15 AM (3):Percolator: ---Training with Cpos selected by cross validation, Cneg selected by cross validation, fdr=0.01
09/13/2013 10:15 AM (3):Percolator: Reading in data and feature calculation took 4.922 cpu seconds or 5 seconds wall time
09/13/2013 10:15 AM (3):Percolator: Estimating 188 over q=0.01 in initial direction
09/13/2013 10:15 AM (3):Percolator: A
09/13/2013 10:15 AM (3):Percolator: selecting cneg by cross validation
09/13/2013 10:15 AM (3):Percolator: selecting cpos by cross validation
09/13/2013 10:15 AM (3):Percolator: baFeatureNames::getNumFeatures=32
09/13/2013 10:15 AM (3):Percolator: Train/test set contains 1240 positives and 1141 negatives, size ratio=1.08677 and pi0=1
09/13/2013 10:15 AM (3):Percolator: e6e22773-e9a6-4a26-9694-1ca77a797099 Delta Cn From Second PSM Binomial Score b8754504-e95e-476b-b9a4-454d4bb53aeb 1d91a87b-953a-4887-9f22-f75a497a3538 Delta Mass [Da] Delta Mass [ppm] Absolute Delta Mass [Da] Absolute Delta Mass [ppm] Peptide Length Is z=1 Is z=2 Is z=3 Is z=4 Is z=5 Is z>5 041eb6d5-e486-44a0-9bc1-19e25811c686 Log Peptides Matched Log Total Intensity Fraction Matched Intensity [%] Fragment Coverage Series A, B, C [%] Fragment Coverage Series X, Y, Z [%] Log Matched Fragment Series Intensities A, B, C Log Matched Fragment Series Intensities X, Y, Z Longest Sequence Series A, B, C Longest Sequence Series X, Y, Z IQR Fragment Delta Mass [Da] IQR Fragment Delta Mass [ppm] Mean Fragment Delta Mass [Da] Mean Fragment Delta Mass [ppm] Mean Absolute Fragment Delta Mass [Da] Mean Absolute Fragment Delta Mass [ppm] end of FeatureNames::setFromXml
09/13/2013 10:15 AM (3):Percolator: in FeatureNames::setFromXml
09/13/2013 10:15 AM (3):Percolator: enzyme=Trypsin
09/13/2013 10:15 AM (3):Percolator: Hyperparameters fdr=0.01, Cpos=0, Cneg=0, maxNiter=10
09/13/2013 10:15 AM (3):Percolator: Started Fri Sep 13 10:15:40 2013 on unknown_host
09/13/2013 10:15 AM (3):Percolator: C:\Program Files\Thermo\Discoverer 1.3\Tools\Percolator\percolator.exe -E C:\Documents and Settings\All Users\Application Data\Thermo\Discoverer 1.3\Scratch\b8649b54-1599-4cad-98bc-b2973cd5597c\input.xml -X C:\Documents and Settings\All Users\Application Data\Thermo\Discoverer 1.3\Scratch\b8649b54-1599-4cad-98bc-b2973cd5597c\output.xml -Z
09/13/2013 10:15 AM (3):Percolator: Issued command:
09/13/2013 10:15 AM (3):Percolator: Department of Genome Sciences at the University of Washington.
09/13/2013 10:15 AM (3):Percolator: Written by Lukas K+ñll (lukall@u.washington.edu) in the
09/13/2013 10:15 AM (3):Percolator: Copyright (c) 2006-9 University of Washington. All rights reserved.
09/13/2013 10:15 AM (3):Percolator: Percolator version 1.17, Build Date Nov 30 2010 15:27:43
09/13/2013 10:15 AM (3):Percolator: Starting Percolator
09/13/2013 10:15 AM (3):Percolator: The input file contains 1240 peptides, 1141 decoy peptides and 32 features.
09/13/2013 10:15 AM (3):Percolator: Creating input file for Mascot (2) took 17.8 s.
09/13/2013 10:15 AM (3):Percolator: Start calculating features for peptides of Mascot (2)
09/13/2013 10:15 AM (2):Mascot: Used mascot server http://194.199.86.179/mascot/ with Mascot version 2.2.7
09/13/2013 10:15 AM (2):Mascot: Sending 905 peptide hits (3244 peptides) to result file
09/13/2013 10:15 AM (2):Mascot: Sending 759 decoy peptide hits (2467 peptides) to result file
09/13/2013 10:15 AM (2):Mascot: Reading decoy results
09/13/2013 10:15 AM (2):Mascot: Start translating results
09/13/2013 10:15 AM (2):Mascot: Start mapping modifications
09/13/2013 10:15 AM (2):Mascot: Received 385 proteins from Mascot server
09/13/2013 10:15 AM (2):Mascot: Start mapping 385 proteins
09/13/2013 10:14 AM (2):Mascot: Start parsing results
09/13/2013 10:14 AM (2):Mascot: Received Mascot result file (filename=../data/20130913/F008072.dat)
09/13/2013 10:14 AM (2):Mascot: Mascot Server completed
09/13/2013 10:13 AM (2):Mascot: Mascot result on server (filename=../data/20130913/F008072.dat)
09/13/2013 10:13 AM (2):Mascot: Start searching 3799 spectra
09/13/2013 10:13 AM (2):Mascot: Receiving spectra 3001 to 3800.
09/13/2013 10:12 AM (2):Mascot: Receiving spectra 2001 to 3001.
09/13/2013 10:12 AM (2):Mascot: Receiving spectra 1001 to 2001.
09/13/2013 10:12 AM (2):Mascot: Use mascot server http://194.199.86.179/mascot/ with Mascot version 2.2.7
09/13/2013 10:12 AM (2):Mascot: Receiving spectra 1 to 1001.
09/13/2013 10:11 AM (1):Spectrum Selector: Reading from File 1 of 1:F:\Cytosol\M15F15 022012\Raw files\F_02_23_E1.raw (9187 spectra total)


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