Multiprocessor-enabled deconvolution (using 8 processors) decon3d: 3d deconvolution... Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 ChI Sel? Proc? WPass WavI WavO 0 0 1 0 457 457 1 1 1 0 617 617 2 2 1 0 525 525 3 0 0 0 0 0 4 0 0 0 0 0 5 0 0 0 0 0 6 0 0 0 0 -146458491847490739771363295232 7 0 0 0 0 0 8 0 0 0 -146801600106670116714009591808 -146801600106670116714009591808 9 0 0 0 0 0 10 0 0 0 0 0 11 0 0 0 0 0 Passed camera intensity offset 100.000000 is greater than min intensity 90.000000... Resetting all channels to default offset. Image Correction Input image file............................. /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_10_R3D.dv Output image file............................ /tmp/hepG2_negcon_001_10_R3D_D3D.dv_cor_OUP Polynomial order............................. 5 X min and max for normalization.............. 0 895 Y min and max for normalization.............. 0 895 Normalization................................ on Type of normalization........................ 1 Camera Intensity Offset (bias)............... 0.00 Bleach correction............................ on Z-line correction............................ on Intensity offset(s).......................... 0.00 50.00 50.00 Photosensor reference value(s)............... 3.7960e+03 5.7415e+05 6.2904e+05 Correcting time point 1, wavelength 1, panel 1... Reading 3D image data (18 sections)... Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 109.53 109.53 3796.0 3796.0 1.0000/1.0000 2 109.68 109.53 3552.0 3547.2 1.0687/0.9987 3 109.64 109.53 3212.0 3208.7 1.1818/0.9990 4 109.75 109.53 3348.0 3341.2 1.1338/0.9980 5 109.68 109.53 3673.0 3668.0 1.0335/0.9986 6 109.84 109.53 3629.0 3618.8 1.0460/0.9972 7 109.83 109.53 3248.0 3239.3 1.1687/0.9973 8 109.97 109.53 3176.0 3163.2 1.1952/0.9960 9 110.00 109.53 3562.0 3546.9 1.0657/0.9958 10 110.00 109.53 3821.0 3804.6 0.9935/0.9957 11 110.11 109.53 3515.0 3496.5 1.0799/0.9947 12 110.19 109.53 3210.0 3190.9 1.1826/0.9940 13 110.12 109.53 3352.0 3334.1 1.1325/0.9946 14 110.19 109.53 3717.0 3694.6 1.0213/0.9940 15 110.30 109.53 3612.0 3586.9 1.0509/0.9930 16 110.22 109.53 3218.0 3197.9 1.1796/0.9938 17 110.33 109.53 3178.0 3154.9 1.1945/0.9927 18 110.40 109.53 3592.0 3563.6 1.0568/0.9921 Correcting time point 1, wavelength 2, panel 1... Reading 3D image data (18 sections)... Photobleaching correction disabled. Best-fit slope is <0 (-1.05780e-05). Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 117.23 117.23 574148.0 574148.0 1.0000/1.0000 2 117.12 117.22 574130.0 574639.6 1.0000/1.0009 3 117.00 117.22 572862.0 573967.8 1.0022/1.0019 4 116.98 117.22 573187.0 574385.6 1.0017/1.0021 5 116.94 117.22 572982.0 574372.7 1.0020/1.0024 6 117.00 117.22 574620.0 575699.8 0.9992/1.0019 7 117.00 117.22 574076.0 575136.9 1.0001/1.0018 8 117.03 117.22 572914.0 573844.7 1.0022/1.0016 9 117.05 117.22 573961.0 574749.7 1.0003/1.0014 10 117.10 117.21 574660.0 575213.4 0.9991/1.0010 11 117.13 117.21 574778.0 575202.6 0.9989/1.0007 12 117.13 117.21 573103.0 573482.5 1.0018/1.0007 13 117.18 117.21 573004.0 573174.6 1.0020/1.0003 14 117.22 117.21 572844.0 572807.8 1.0023/0.9999 15 117.28 117.21 573234.0 572901.9 1.0016/0.9994 16 117.35 117.21 574242.0 573563.2 0.9998/0.9988 17 117.39 117.21 573535.0 572613.6 1.0011/0.9984 18 117.47 117.20 574285.0 572998.4 0.9998/0.9978 Correcting time point 1, wavelength 3, panel 1... Reading 3D image data (18 sections)... Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 106.86 106.86 629043.0 629043.0 1.0000/1.0000 2 106.72 106.86 626353.0 627183.5 1.0043/1.0013 3 106.66 106.86 628359.0 629544.4 1.0011/1.0019 4 106.64 106.86 625556.0 626872.1 1.0056/1.0021 5 106.58 106.86 626770.0 628389.2 1.0036/1.0026 6 106.57 106.86 624826.0 626509.4 1.0067/1.0027 7 106.59 106.86 625775.0 627371.4 1.0052/1.0026 8 106.64 106.86 627988.0 629298.2 1.0017/1.0021 9 106.64 106.86 629400.0 630703.1 0.9994/1.0021 10 106.65 106.86 628049.0 629293.9 1.0016/1.0020 11 106.65 106.86 628353.0 629607.4 1.0011/1.0020 12 106.66 106.86 627253.0 628452.7 1.0029/1.0019 13 106.68 106.86 628318.0 629393.2 1.0012/1.0017 14 106.71 106.86 629178.0 630064.5 0.9998/1.0014 15 106.73 106.86 626949.0 627742.0 1.0033/1.0013 16 106.72 106.86 627711.0 628529.2 1.0021/1.0013 17 106.74 106.86 627371.0 628060.6 1.0027/1.0011 18 106.77 106.86 626391.0 626927.1 1.0042/1.0009 ---------------------------------------------------- Deconvolution Results: Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 Transferring field 3: COR_BlchFitSlp f 8 0.000019 Transferring field 4: COR_BlchFitInt f 8 4.672324 Transferring field 5: COR_BlchFitChi f 8 0.000000 Opening OTF file (/usr/local/otf/Olympus_100X_140_10002.otf)... decon3d: 3D iterative constrained deconvolution Input image file............................. /tmp/hepG2_negcon_001_10_R3D_D3D.dv_cor_OUP Output image file............................ /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_10_R3D_D3D.dv Method (1=add,2=ratio,3=enh add,4=enh ratio). 4 Values subtracted from image................. 0.00 0.00 0.00 Intensity scaling factor..................... 1.0000 Border roll-off size (pixels)................ 28 Pre-filter cut-off values (microns).......... 0.2000, 0.0500 Sigma for smoothing.......................... 0.3050 Sigma for smoothing Wiener filter............ 0.1100 S:N value for Wiener filter.................. 0.1010 Z padding.................................... 64 Number of iterations before smoothing........ 5 Maximum number of iterations................. 10 Autoscaling.................................. enabled Allocating image memory (nx=896,ny=896,sOpt->nzo=18,total=13.8 MP)... Generating pre-filter and smoothing function... Wavelength 457.0 nm processed with OTF measured at 617.0 nm (scale=1.350)... Preparing OTF (64 128 129)... Deconvolving wavelength 457.0, time point 1 (3D)... Z noise filter threshold=1096.00 Prefiltering wavelength 457.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01405591 1.53 1.000 2 0.01055488 1.15 0.751 3 0.01020902 1.11 0.726 4 0.01005863 1.10 0.716 5 0.00995108 1.09 0.708 6 0.01003391 1.10 0.714 7 0.00991494 1.08 0.705 8 0.00982077 1.07 0.699 9 0.00973796 1.06 0.693 10 0.00966439 1.06 0.688 decon3d: finished iterations (10/10). Wavelength 617.0 nm processed with OTF measured at 617.0 nm (scale=1.000)... Preparing OTF (64 128 129)... Deconvolving wavelength 617.0, time point 1 (3D)... Z noise filter threshold=1104.00 Prefiltering wavelength 617.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01289226 1.50 1.000 2 0.01099652 1.28 0.853 3 0.01076871 1.26 0.835 4 0.01065318 1.24 0.826 5 0.01060856 1.24 0.823 6 0.01065027 1.24 0.826 7 0.01059415 1.24 0.822 8 0.01055414 1.23 0.819 9 0.01052021 1.23 0.816 10 0.01049172 1.22 0.814 decon3d: finished iterations (10/10). Wavelength 525.0 nm processed with OTF measured at 617.0 nm (scale=1.175)... Preparing OTF (64 128 129)... Deconvolving wavelength 525.0, time point 1 (3D)... Z noise filter threshold=1104.00 Prefiltering wavelength 525.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01087658 1.16 1.000 2 0.01039250 1.10 0.955 3 0.01024314 1.09 0.942 4 0.01014390 1.08 0.933 5 0.01007833 1.07 0.927 6 0.01012668 1.08 0.931 7 0.01005613 1.07 0.925 8 0.00999873 1.06 0.919 9 0.00994774 1.06 0.915 10 0.00990247 1.05 0.910 decon3d: finished iterations (10/10). Total deconvolution time: 00:00:11