Multiprocessor-enabled deconvolution (using 8 processors) decon3d: 3d deconvolution... Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 ChI Sel? Proc? WPass WavI WavO 0 0 1 0 457 457 1 1 1 0 617 617 2 2 1 0 525 525 3 0 0 0 0 0 4 0 0 0 0 0 5 0 0 0 0 0 6 0 0 0 0 -2217573734396981192555478646784 7 0 0 0 0 0 8 0 0 0 -2223063466543851223637819392000 -2223063466543851223637819392000 9 0 0 0 0 0 10 0 0 0 0 0 11 0 0 0 0 0 Passed camera intensity offset 100.000000 is greater than min intensity 89.000000... Resetting all channels to default offset. Image Correction Input image file............................. /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_09_R3D.dv Output image file............................ /tmp/hepG2_negcon_001_09_R3D_D3D.dv_cor_BHC Polynomial order............................. 5 X min and max for normalization.............. 0 895 Y min and max for normalization.............. 0 895 Normalization................................ on Type of normalization........................ 1 Camera Intensity Offset (bias)............... 0.00 Bleach correction............................ on Z-line correction............................ on Intensity offset(s).......................... 0.00 50.00 50.00 Photosensor reference value(s)............... 3.5440e+03 5.7289e+05 6.2839e+05 Correcting time point 1, wavelength 1, panel 1... Reading 3D image data (18 sections)... Photobleaching correction disabled. Best-fit slope is <0 (-2.37751e-04). Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 108.12 108.12 3544.0 3544.0 1.0000/1.0000 2 108.19 108.09 3230.0 3227.0 1.0972/0.9991 3 108.17 108.06 3351.0 3347.7 1.0576/0.9990 4 108.17 108.04 3721.0 3716.6 0.9524/0.9988 5 108.25 108.01 3680.0 3671.9 0.9630/0.9978 6 108.28 107.99 3279.0 3270.1 1.0808/0.9973 7 108.35 107.96 3222.0 3210.4 1.0999/0.9964 8 108.39 107.94 3602.0 3587.0 0.9839/0.9958 9 108.47 107.91 3871.0 3851.0 0.9155/0.9948 10 108.47 107.88 3549.0 3529.9 0.9986/0.9946 11 108.53 107.86 3212.0 3192.2 1.1034/0.9938 12 108.59 107.83 3391.0 3367.2 1.0451/0.9930 13 108.65 107.81 3783.0 3753.5 0.9368/0.9922 14 108.75 107.78 3661.0 3628.3 0.9680/0.9911 15 108.80 107.76 3283.0 3251.6 1.0795/0.9904 16 108.86 107.73 3215.0 3181.6 1.1023/0.9896 17 108.88 107.70 3627.0 3587.8 0.9771/0.9892 18 108.99 107.68 3832.0 3786.0 0.9248/0.9880 Correcting time point 1, wavelength 2, panel 1... Reading 3D image data (18 sections)... Photobleaching correction disabled. Best-fit slope is <0 (-1.46693e-05). Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 123.85 123.85 572893.0 572893.0 1.0000/1.0000 2 123.72 123.85 573170.0 573794.2 0.9995/1.0011 3 123.65 123.85 573173.0 574117.1 0.9995/1.0016 4 123.63 123.85 574029.0 575035.3 0.9980/1.0018 5 123.62 123.85 573852.0 574914.8 0.9983/1.0019 6 123.65 123.84 573476.0 574373.7 0.9990/1.0016 7 123.70 123.84 573916.0 574584.1 0.9982/1.0012 8 123.75 123.84 573892.0 574315.5 0.9983/1.0007 9 123.68 123.84 572641.0 573376.3 1.0004/1.0013 10 123.75 123.84 573948.0 574330.1 0.9982/1.0007 11 123.85 123.84 574446.0 574357.5 0.9973/0.9998 12 123.92 123.83 573866.0 573487.5 0.9983/0.9993 13 124.02 123.83 573989.0 573119.2 0.9981/0.9985 14 124.11 123.83 573989.0 572688.3 0.9981/0.9977 15 124.18 123.83 572691.0 571066.8 1.0004/0.9972 16 124.34 123.83 573905.0 571535.4 0.9982/0.9959 17 124.38 123.82 573370.0 570794.6 0.9992/0.9955 18 124.48 123.82 574156.0 571108.7 0.9978/0.9947 Correcting time point 1, wavelength 3, panel 1... Reading 3D image data (18 sections)... Photobleaching correction disabled. Best-fit slope is <0 (-1.99917e-05). Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 109.39 109.39 628390.0 628390.0 1.0000/1.0000 2 109.25 109.39 628629.0 629446.1 0.9996/1.0013 3 109.15 109.39 626407.0 627777.5 1.0032/1.0022 4 109.09 109.38 627986.0 629656.8 1.0006/1.0027 5 109.08 109.38 628779.0 630490.2 0.9994/1.0027 6 109.12 109.38 631678.0 633188.9 0.9948/1.0024 7 109.09 109.38 629376.0 631000.2 0.9984/1.0026 8 109.13 109.37 630811.0 632242.9 0.9962/1.0023 9 109.12 109.37 629962.0 631396.1 0.9975/1.0023 10 109.12 109.37 627011.0 628425.9 1.0022/1.0023 11 109.10 109.37 625625.0 627139.8 1.0044/1.0024 12 109.14 109.37 629231.0 630509.9 0.9987/1.0020 13 109.23 109.36 628551.0 629318.9 0.9997/1.0012 14 109.25 109.36 630314.0 630970.2 0.9969/1.0010 15 109.25 109.36 628270.0 628905.6 1.0002/1.0010 16 109.27 109.36 628354.0 628878.6 1.0001/1.0008 17 109.34 109.35 630850.0 630928.1 0.9961/1.0001 18 109.38 109.35 628972.0 628799.9 0.9991/0.9997 ---------------------------------------------------- Deconvolution Results: Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 Opening OTF file (/usr/local/otf/Olympus_100X_140_10002.otf)... decon3d: 3D iterative constrained deconvolution Input image file............................. /tmp/hepG2_negcon_001_09_R3D_D3D.dv_cor_BHC Output image file............................ /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_09_R3D_D3D.dv Method (1=add,2=ratio,3=enh add,4=enh ratio). 4 Values subtracted from image................. 0.00 0.00 0.00 Intensity scaling factor..................... 1.0000 Border roll-off size (pixels)................ 28 Pre-filter cut-off values (microns).......... 0.2000, 0.0500 Sigma for smoothing.......................... 0.3050 Sigma for smoothing Wiener filter............ 0.1100 S:N value for Wiener filter.................. 0.1010 Z padding.................................... 64 Number of iterations before smoothing........ 5 Maximum number of iterations................. 10 Autoscaling.................................. enabled Allocating image memory (nx=896,ny=896,sOpt->nzo=18,total=13.8 MP)... Generating pre-filter and smoothing function... Wavelength 457.0 nm processed with OTF measured at 617.0 nm (scale=1.350)... Preparing OTF (64 128 129)... Deconvolving wavelength 457.0, time point 1 (3D)... Z noise filter threshold=1096.00 Prefiltering wavelength 457.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01233352 1.32 1.000 2 0.01048293 1.12 0.850 3 0.01020109 1.09 0.827 4 0.01006048 1.08 0.816 5 0.00995452 1.07 0.807 6 0.01003190 1.08 0.813 7 0.00991738 1.06 0.804 8 0.00982544 1.05 0.797 9 0.00974410 1.05 0.790 10 0.00967157 1.04 0.784 decon3d: finished iterations (10/10). Wavelength 617.0 nm processed with OTF measured at 617.0 nm (scale=1.000)... Preparing OTF (64 128 129)... Deconvolving wavelength 617.0, time point 1 (3D)... Z noise filter threshold=1104.00 Prefiltering wavelength 617.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01300572 1.60 1.000 2 0.01131878 1.40 0.870 3 0.01105631 1.36 0.850 4 0.01092031 1.35 0.840 5 0.01087028 1.34 0.836 6 0.01091759 1.35 0.839 7 0.01085440 1.34 0.835 8 0.01080969 1.33 0.831 9 0.01077180 1.33 0.828 10 0.01074001 1.32 0.826 decon3d: finished iterations (10/10). Wavelength 525.0 nm processed with OTF measured at 617.0 nm (scale=1.175)... Preparing OTF (64 128 129)... Deconvolving wavelength 525.0, time point 1 (3D)... Z noise filter threshold=1104.00 Prefiltering wavelength 525.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01096566 1.19 1.000 2 0.01052109 1.14 0.959 3 0.01036886 1.13 0.946 4 0.01026770 1.12 0.936 5 0.01020233 1.11 0.930 6 0.01025145 1.11 0.935 7 0.01017984 1.11 0.928 8 0.01012214 1.10 0.923 9 0.01007100 1.10 0.918 10 0.01002573 1.09 0.914 decon3d: finished iterations (10/10). Total deconvolution time: 00:00:12