Multiprocessor-enabled deconvolution (using 8 processors) decon3d: 3d deconvolution... Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 ChI Sel? Proc? WPass WavI WavO 0 0 1 0 457 457 1 1 1 0 617 617 2 2 1 0 525 525 3 0 0 0 0 0 4 0 0 0 0 0 5 0 0 0 0 0 6 0 0 0 0 150601440736758026670318595080192 7 0 0 0 0 0 8 0 0 0 150952783594157708659588402774016 150952783594157708659588402774016 9 0 0 0 0 0 10 0 0 0 0 0 11 0 0 0 0 0 Passed camera intensity offset 100.000000 is greater than min intensity 90.000000... Resetting all channels to default offset. Image Correction Input image file............................. /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_08_R3D.dv Output image file............................ /tmp/hepG2_negcon_001_08_R3D_D3D.dv_cor_PUO Polynomial order............................. 5 X min and max for normalization.............. 0 895 Y min and max for normalization.............. 0 895 Normalization................................ on Type of normalization........................ 1 Camera Intensity Offset (bias)............... 0.00 Bleach correction............................ on Z-line correction............................ on Intensity offset(s).......................... 0.00 50.00 50.00 Photosensor reference value(s)............... 3.2100e+03 5.7292e+05 6.2789e+05 Correcting time point 1, wavelength 1, panel 1... Reading 3D image data (18 sections)... Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 111.13 111.13 3210.0 3210.0 1.0000/1.0000 2 111.22 111.13 2971.0 2968.6 1.0804/0.9992 3 111.22 111.13 2603.0 2600.9 1.2332/0.9992 4 111.36 111.13 2716.0 2710.3 1.1819/0.9979 5 111.43 111.13 3078.0 3069.7 1.0429/0.9973 6 111.53 111.13 3049.0 3038.0 1.0528/0.9964 7 111.58 111.13 2650.0 2639.4 1.2113/0.9960 8 111.72 111.13 2583.0 2569.3 1.2427/0.9947 9 111.80 111.13 2965.0 2947.2 1.0826/0.9940 10 111.74 111.13 3207.0 3189.6 1.0009/0.9946 11 111.88 111.13 2912.0 2892.5 1.1023/0.9933 12 112.00 111.13 2581.0 2560.9 1.2437/0.9922 13 111.98 111.13 2761.0 2739.9 1.1626/0.9924 14 112.14 111.13 3115.0 3087.0 1.0305/0.9910 15 112.17 111.13 3011.0 2983.2 1.0661/0.9908 16 112.20 111.13 2615.0 2589.9 1.2275/0.9904 17 112.15 111.13 2587.0 2563.4 1.2408/0.9909 18 112.33 111.13 2986.0 2953.9 1.0750/0.9893 Correcting time point 1, wavelength 2, panel 1... Reading 3D image data (18 sections)... Photobleaching correction disabled. Best-fit slope is <0 (-1.38957e-05). Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 118.29 118.29 572923.0 572923.0 1.0000/1.0000 2 118.21 118.29 573830.0 574176.9 0.9984/1.0006 3 117.97 118.28 573827.0 575362.1 0.9984/1.0027 4 117.90 118.28 572876.0 574723.7 1.0001/1.0032 5 117.88 118.28 573978.0 575942.0 0.9982/1.0034 6 117.88 118.28 573066.0 574989.6 0.9998/1.0034 7 117.89 118.28 573234.0 575128.9 0.9995/1.0033 8 117.91 118.28 571996.0 573758.4 1.0016/1.0031 9 117.95 118.27 572510.0 574087.8 1.0007/1.0028 10 118.01 118.27 573618.0 574917.9 0.9988/1.0023 11 118.01 118.27 572794.0 574053.4 1.0002/1.0022 12 118.05 118.27 573209.0 574265.0 0.9995/1.0018 13 118.12 118.27 572936.0 573673.1 1.0000/1.0013 14 118.15 118.27 573891.0 574436.9 0.9983/1.0010 15 118.18 118.26 573064.0 573468.9 0.9998/1.0007 16 118.27 118.26 573508.0 573490.6 0.9990/1.0000 17 118.30 118.26 573616.0 573440.8 0.9988/0.9997 18 118.35 118.26 573643.0 573193.3 0.9987/0.9992 Correcting time point 1, wavelength 3, panel 1... Reading 3D image data (18 sections)... Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 108.29 108.29 627892.0 627892.0 1.0000/1.0000 2 108.02 108.29 629485.0 631054.3 0.9975/1.0025 3 107.85 108.29 628448.0 631013.4 0.9991/1.0041 4 107.77 108.29 628302.0 631298.8 0.9993/1.0048 5 107.73 108.29 626658.0 629921.9 1.0020/1.0052 6 107.66 108.29 625524.0 629155.2 1.0038/1.0058 7 107.69 108.29 629624.0 633124.5 0.9972/1.0056 8 107.64 108.29 626555.0 630343.2 1.0021/1.0060 9 107.64 108.29 628675.0 632474.6 0.9988/1.0060 10 107.62 108.29 627040.0 630937.8 1.0014/1.0062 11 107.63 108.29 628216.0 632037.1 0.9995/1.0061 12 107.62 108.29 625833.0 629724.9 1.0033/1.0062 13 107.66 108.29 627745.0 631397.2 1.0002/1.0058 14 107.67 108.29 630730.0 634333.0 0.9955/1.0057 15 107.65 108.29 629185.0 632901.1 0.9979/1.0059 16 107.69 108.29 628202.0 631708.6 0.9995/1.0056 17 107.69 108.29 629271.0 632756.4 0.9978/1.0055 18 107.72 108.29 628228.0 631522.8 0.9995/1.0052 ---------------------------------------------------- Deconvolution Results: Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 Transferring field 3: COR_BlchFitSlp f 8 0.000002 Transferring field 4: COR_BlchFitInt f 8 4.678705 Transferring field 5: COR_BlchFitChi f 8 0.000000 Opening OTF file (/usr/local/otf/Olympus_100X_140_10002.otf)... decon3d: 3D iterative constrained deconvolution Input image file............................. /tmp/hepG2_negcon_001_08_R3D_D3D.dv_cor_PUO Output image file............................ /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_08_R3D_D3D.dv Method (1=add,2=ratio,3=enh add,4=enh ratio). 4 Values subtracted from image................. 0.00 0.00 0.00 Intensity scaling factor..................... 1.0000 Border roll-off size (pixels)................ 28 Pre-filter cut-off values (microns).......... 0.2000, 0.0500 Sigma for smoothing.......................... 0.3050 Sigma for smoothing Wiener filter............ 0.1100 S:N value for Wiener filter.................. 0.1010 Z padding.................................... 64 Number of iterations before smoothing........ 5 Maximum number of iterations................. 10 Autoscaling.................................. enabled Allocating image memory (nx=896,ny=896,sOpt->nzo=18,total=13.8 MP)... Generating pre-filter and smoothing function... Wavelength 457.0 nm processed with OTF measured at 617.0 nm (scale=1.350)... Preparing OTF (64 128 129)... Deconvolving wavelength 457.0, time point 1 (3D)... Z noise filter threshold=1096.00 Prefiltering wavelength 457.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01343969 1.48 1.000 2 0.01078528 1.19 0.802 3 0.01040222 1.15 0.774 4 0.01023093 1.13 0.761 5 0.01011544 1.12 0.753 6 0.01020277 1.13 0.759 7 0.01007715 1.11 0.750 8 0.00997811 1.10 0.742 9 0.00989157 1.09 0.736 10 0.00981511 1.08 0.730 decon3d: finished iterations (10/10). Wavelength 617.0 nm processed with OTF measured at 617.0 nm (scale=1.000)... Preparing OTF (64 128 129)... Deconvolving wavelength 617.0, time point 1 (3D)... Z noise filter threshold=1112.00 Prefiltering wavelength 617.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01192094 1.40 1.000 2 0.01102798 1.30 0.925 3 0.01083155 1.27 0.909 4 0.01071845 1.26 0.899 5 0.01067364 1.26 0.895 6 0.01071507 1.26 0.899 7 0.01065904 1.25 0.894 8 0.01061921 1.25 0.891 9 0.01058532 1.25 0.888 10 0.01055683 1.24 0.886 decon3d: finished iterations (10/10). Wavelength 525.0 nm processed with OTF measured at 617.0 nm (scale=1.175)... Preparing OTF (64 128 129)... Deconvolving wavelength 525.0, time point 1 (3D)... Z noise filter threshold=1104.00 Prefiltering wavelength 525.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01081055 1.16 1.000 2 0.01045995 1.12 0.968 3 0.01031340 1.11 0.954 4 0.01021431 1.10 0.945 5 0.01014905 1.09 0.939 6 0.01019659 1.10 0.943 7 0.01012674 1.09 0.937 8 0.01006933 1.08 0.931 9 0.01001835 1.08 0.927 10 0.00997314 1.07 0.923 decon3d: finished iterations (10/10). Total deconvolution time: 00:00:11