Multiprocessor-enabled deconvolution (using 8 processors) decon3d: 3d deconvolution... Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 ChI Sel? Proc? WPass WavI WavO 0 0 1 0 457 457 1 1 1 0 617 617 2 2 1 0 525 525 3 0 0 0 0 0 4 0 0 0 0 0 5 0 0 0 0 0 6 0 0 0 0 -0 7 0 0 0 0 0 8 0 0 0 -0 -0 9 0 0 0 0 0 10 0 0 0 0 0 11 0 0 0 0 0 Passed camera intensity offset 100.000000 is greater than min intensity 89.000000... Resetting all channels to default offset. Image Correction Input image file............................. /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_07_R3D.dv Output image file............................ /tmp/hepG2_negcon_001_07_R3D_D3D.dv_cor_CHB Polynomial order............................. 5 X min and max for normalization.............. 0 895 Y min and max for normalization.............. 0 895 Normalization................................ on Type of normalization........................ 1 Camera Intensity Offset (bias)............... 0.00 Bleach correction............................ on Z-line correction............................ on Intensity offset(s).......................... 0.00 50.00 50.00 Photosensor reference value(s)............... 3.4780e+03 5.7392e+05 6.2911e+05 Correcting time point 1, wavelength 1, panel 1... Reading 3D image data (18 sections)... Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 111.81 111.81 3478.0 3478.0 1.0000/1.0000 2 111.82 111.81 3470.0 3469.8 1.0023/0.9999 3 111.95 111.81 3133.0 3129.1 1.1101/0.9988 4 112.02 111.81 2983.0 2977.4 1.1659/0.9981 5 112.22 111.81 3398.0 3385.5 1.0235/0.9963 6 112.21 111.81 3622.0 3609.1 0.9602/0.9964 7 112.37 111.81 3375.0 3358.3 1.0305/0.9950 8 112.41 111.81 3022.0 3005.9 1.1509/0.9947 9 112.53 111.81 3125.0 3104.9 1.1130/0.9936 10 112.63 111.81 3489.0 3463.5 0.9968/0.9927 11 112.73 111.81 3455.0 3426.7 1.0067/0.9918 12 112.86 111.81 3096.0 3067.3 1.1234/0.9907 13 112.98 111.81 2979.0 2948.2 1.1675/0.9897 14 113.01 111.81 3372.0 3336.0 1.0314/0.9893 15 113.05 111.81 3610.0 3570.3 0.9634/0.9890 16 113.19 111.81 3326.0 3285.5 1.0457/0.9878 17 113.22 111.81 3002.0 2964.6 1.1586/0.9875 18 113.38 111.81 3160.0 3116.2 1.1006/0.9861 Correcting time point 1, wavelength 2, panel 1... Reading 3D image data (18 sections)... Photobleaching correction disabled. Best-fit slope is <0 (-2.64795e-06). Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 111.80 111.80 573925.0 573925.0 1.0000/1.0000 2 111.72 111.80 573655.0 574032.5 1.0005/1.0007 3 111.72 111.80 573700.0 574070.2 1.0004/1.0006 4 111.69 111.80 572879.0 573415.9 1.0018/1.0009 5 111.76 111.80 574419.0 574583.9 0.9991/1.0003 6 111.76 111.80 574834.0 575033.8 0.9984/1.0003 7 111.74 111.80 573289.0 573551.7 1.0011/1.0005 8 111.81 111.80 574197.0 574140.4 0.9995/0.9999 9 111.83 111.79 573482.0 573307.4 1.0008/0.9997 10 111.77 111.79 573460.0 573604.9 1.0008/1.0003 11 111.83 111.79 573963.0 573767.0 0.9999/0.9997 12 111.75 111.79 573061.0 573270.0 1.0015/1.0004 13 111.83 111.79 573899.0 573696.1 1.0000/0.9996 14 111.81 111.79 573954.0 573848.3 0.9999/0.9998 15 111.83 111.79 574706.0 574498.0 0.9986/0.9996 16 111.88 111.79 575036.0 574567.0 0.9981/0.9992 17 111.91 111.79 575033.0 574406.0 0.9981/0.9989 18 111.98 111.79 574626.0 573649.2 0.9988/0.9983 Correcting time point 1, wavelength 3, panel 1... Reading 3D image data (18 sections)... Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 105.35 105.35 629112.0 629112.0 1.0000/1.0000 2 105.21 105.35 626712.0 627543.8 1.0038/1.0013 3 105.16 105.35 626210.0 627371.7 1.0046/1.0019 4 105.14 105.35 630522.0 631806.1 0.9978/1.0020 5 105.12 105.35 627791.0 629179.8 1.0021/1.0022 6 105.06 105.35 629449.0 631216.7 0.9995/1.0028 7 105.07 105.35 630189.0 631895.2 0.9983/1.0027 8 105.02 105.35 623710.0 625678.9 1.0087/1.0032 9 105.06 105.35 627917.0 629682.8 1.0019/1.0028 10 105.04 105.35 627888.0 629778.7 1.0019/1.0030 11 105.06 105.35 629160.0 630902.8 0.9999/1.0028 12 105.05 105.35 628044.0 629865.9 1.0017/1.0029 13 105.09 105.35 627248.0 628805.8 1.0030/1.0025 14 105.10 105.35 630128.0 631655.5 0.9984/1.0024 15 105.11 105.35 629915.0 631357.2 0.9987/1.0023 16 105.11 105.35 631638.0 633125.1 0.9960/1.0024 17 105.10 105.35 626809.0 628290.8 1.0037/1.0024 18 105.12 105.35 628419.0 629787.0 1.0011/1.0022 ---------------------------------------------------- Deconvolution Results: Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 Transferring field 3: COR_BlchFitSlp f 8 0.000005 Transferring field 4: COR_BlchFitInt f 8 4.655724 Transferring field 5: COR_BlchFitChi f 8 0.000000 Opening OTF file (/usr/local/otf/Olympus_100X_140_10002.otf)... decon3d: 3D iterative constrained deconvolution Input image file............................. /tmp/hepG2_negcon_001_07_R3D_D3D.dv_cor_CHB Output image file............................ /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_07_R3D_D3D.dv Method (1=add,2=ratio,3=enh add,4=enh ratio). 4 Values subtracted from image................. 0.00 0.00 0.00 Intensity scaling factor..................... 1.0000 Border roll-off size (pixels)................ 28 Pre-filter cut-off values (microns).......... 0.2000, 0.0500 Sigma for smoothing.......................... 0.3050 Sigma for smoothing Wiener filter............ 0.1100 S:N value for Wiener filter.................. 0.1010 Z padding.................................... 64 Number of iterations before smoothing........ 5 Maximum number of iterations................. 10 Autoscaling.................................. enabled Allocating image memory (nx=896,ny=896,sOpt->nzo=18,total=13.8 MP)... Generating pre-filter and smoothing function... Wavelength 457.0 nm processed with OTF measured at 617.0 nm (scale=1.350)... Preparing OTF (64 128 129)... Deconvolving wavelength 457.0, time point 1 (3D)... Z noise filter threshold=1096.00 Prefiltering wavelength 457.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01406074 1.56 1.000 2 0.01078053 1.20 0.767 3 0.01035437 1.15 0.736 4 0.01018255 1.13 0.724 5 0.01006873 1.12 0.716 6 0.01015774 1.13 0.722 7 0.01003366 1.12 0.714 8 0.00993549 1.11 0.707 9 0.00985001 1.10 0.701 10 0.00977459 1.09 0.695 decon3d: finished iterations (10/10). Wavelength 617.0 nm processed with OTF measured at 617.0 nm (scale=1.000)... Preparing OTF (64 128 129)... Deconvolving wavelength 617.0, time point 1 (3D)... Z noise filter threshold=1104.00 Prefiltering wavelength 617.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01114391 1.24 1.000 2 0.01070683 1.19 0.961 3 0.01054882 1.17 0.947 4 0.01044903 1.16 0.938 5 0.01040812 1.16 0.934 6 0.01044568 1.16 0.937 7 0.01039460 1.16 0.933 8 0.01035795 1.15 0.929 9 0.01032658 1.15 0.927 10 0.01030004 1.15 0.924 decon3d: finished iterations (10/10). Wavelength 525.0 nm processed with OTF measured at 617.0 nm (scale=1.175)... Preparing OTF (64 128 129)... Deconvolving wavelength 525.0, time point 1 (3D)... Z noise filter threshold=1104.00 Prefiltering wavelength 525.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01061147 1.11 1.000 2 0.01034617 1.08 0.975 3 0.01020473 1.07 0.962 4 0.01010652 1.06 0.952 5 0.01004108 1.05 0.946 6 0.01008844 1.06 0.951 7 0.01001894 1.05 0.944 8 0.00996156 1.04 0.939 9 0.00991042 1.04 0.934 10 0.00986492 1.03 0.930 decon3d: finished iterations (10/10). Total deconvolution time: 00:00:12