# #Tag 0x00FF0408 Resolve3D softWoRx-Acquire Version: 7.0.0 Release RC6 Running on host: cn60rt100360 Resolve3D Image File: /data1/Stojic_lab/Claudia_Kutter/10Nove2021_treatments/hepG2_negcon_001_07_R3D.dv Created Wed Nov 10 17:09:19 2021 Optics: Objective: Olympus 100X/1.40, Plan Apo, IX70 Lens ID: 10002 Aux Magn: 1.00 Image Properties: XY Dimensions: 896 x 896 ZWT Dimensions (expected): 18 x 3 x 1 Pixel Size: 0.06450 0.06450 0.300 Binning: 1x1 Flat-field Calibration: Off Camera: Type: CoolSNAP_HQ2 / HQ2-ICX285 Gain: 1.00X Speed: 10000KHz Temp Setting: -25.0C Resolve3D Macro: -------------------------------------------------------- # Created by DeltaVision Experiment Designer (version: 7.0.0 Release RC6) # Experiment Macro Name: Resolve3D # Open image files for Point Visiting OPF2 7-25 FIRSTPOINT # Starting Scan From Current Focus (Middle of sample) # Move to start of sectioning from initial point. Remove backlash. FOCUS 10.550 FOCUS -7.800 FOCUS -0.100 FOCUS -0.100 # Point visiting loop. DO PointList # Select Ex, Em, Nd Filters FILTERS DAPI,DAPI,10% ACTSHUT 17 # Z sectioning loop. FIRST_Z DO 18 CCD 0.200000 WRT2 SECTIONZ -0.300 NEXT_Z ENDDO # Move to start of sectioning from end of scan. Remove backlash. FOCUS 13.400 FOCUS -7.800 FOCUS -0.100 FOCUS -0.100 # Select Ex, Em, Nd Filters FILTERS TRITC,TRITC,100% ACTSHUT 17 # Z sectioning loop. FIRST_Z DO 18 CCD 2.000000 WRT2 SECTIONZ -0.300 NEXT_Z ENDDO # Move to start of sectioning from end of scan. Remove backlash. FOCUS 13.400 FOCUS -7.800 FOCUS -0.100 FOCUS -0.100 # Select Ex, Em, Nd Filters FILTERS FITC,FITC,100% ACTSHUT 17 # Z sectioning loop. FIRST_Z DO 18 CCD 0.300000 WRT2 SECTIONZ -0.300 NEXT_Z ENDDO # Move to start of sectioning from end of scan. Remove backlash. FOCUS 13.400 FOCUS -7.800 FOCUS -0.100 FOCUS -0.100 # Move to next visited point NEXTPOINT # Starting Scan From Current Focus (Middle of sample) # Move to start of sectioning from initial point. Remove backlash. FOCUS 10.550 FOCUS -7.800 FOCUS -0.100 FOCUS -0.100 ENDDO FIRSTPOINT # Close image file(s) CLF2 BEEP # # #KEY Experiment Designer Keys: Don't Modify # #KEY EXPERIMENT_TYPE STANDARD #KEY SCAN_SEQUENCE ZWT #KEY DO_FAST_ACQUISITION F #KEY LAMPS_OFF_AT_END F #KEY DO_Z_SECTIONING T #KEY Z_APPLY_BACKLASH T #KEY Z_BACKLASH 8.0000 #KEY Z_START_POSITION MIDDLE #KEY SECT_SPACING 0.3000 #KEY NUM_Z_SECTIONS 18 #KEY SAMPLE_THICKNESS 5.4000 #KEY CHANNEL 0.200000,DAPI,DAPI,10%,1 #KEY EX_SHUTTERS 2,EX=T,TRANS=F #KEY EM_SHUTTERS 1,CCD1=T #KEY CHANNEL 2.000000,TRITC,TRITC,100%,1 #KEY EX_SHUTTERS 2,EX=T,TRANS=F #KEY EM_SHUTTERS 1,CCD1=T #KEY CHANNEL 0.300000,FITC,FITC,100%,1 #KEY EX_SHUTTERS 2,EX=T,TRANS=F #KEY EM_SHUTTERS 1,CCD1=T #KEY DO_REFERENCE_IMAGE F #KEY DO_CELL_TRACK F #KEY DO_AUTOFOCUS F #KEY DO_ULTIMATEFOCUS F #KEY DO_OAI_SCANNING F #KEY DO_TIME_LAPSE F #KEY DO_POINT_VISITING T #KEY POINT_VISIT_LIST 7-25 #KEY DO_PANEL_COLLECTION F # # -------------------------------------------------------- Experiment Record: ------------------ Image 1. Time: Wed Nov 10 17:09:22 2021 Time Point: 0.000 secs Intensity: Min = 91, Max = 210, Mean = 111.80939 Photosensor reading: 3478.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-86.741) Image 2. Time: Wed Nov 10 17:09:22 2021 Time Point: 0.682 secs Intensity: Min = 90, Max = 216, Mean = 111.81696 Photosensor reading: 3470.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-87.030) Image 3. Time: Wed Nov 10 17:09:23 2021 Time Point: 1.332 secs Intensity: Min = 90, Max = 224, Mean = 111.94836 Photosensor reading: 3133.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-87.330) Image 4. Time: Wed Nov 10 17:09:24 2021 Time Point: 1.984 secs Intensity: Min = 91, Max = 232, Mean = 112.02070 Photosensor reading: 2983.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-87.631) Image 5. Time: Wed Nov 10 17:09:24 2021 Time Point: 2.685 secs Intensity: Min = 90, Max = 234, Mean = 112.22205 Photosensor reading: 3398.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-87.931) Image 6. Time: Wed Nov 10 17:09:25 2021 Time Point: 3.403 secs Intensity: Min = 90, Max = 244, Mean = 112.20982 Photosensor reading: 3622.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-88.231) Image 7. Time: Wed Nov 10 17:09:26 2021 Time Point: 4.105 secs Intensity: Min = 89, Max = 252, Mean = 112.36674 Photosensor reading: 3375.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-88.531) Image 8. Time: Wed Nov 10 17:09:26 2021 Time Point: 4.806 secs Intensity: Min = 91, Max = 267, Mean = 112.40752 Photosensor reading: 3022.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-88.829) Image 9. Time: Wed Nov 10 17:09:27 2021 Time Point: 5.524 secs Intensity: Min = 91, Max = 267, Mean = 112.53487 Photosensor reading: 3125.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-89.129) Image 10. Time: Wed Nov 10 17:09:28 2021 Time Point: 6.259 secs Intensity: Min = 90, Max = 275, Mean = 112.63319 Photosensor reading: 3489.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-89.432) Image 11. Time: Wed Nov 10 17:09:29 2021 Time Point: 6.974 secs Intensity: Min = 91, Max = 279, Mean = 112.73436 Photosensor reading: 3455.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-89.732) Image 12. Time: Wed Nov 10 17:09:29 2021 Time Point: 7.675 secs Intensity: Min = 90, Max = 273, Mean = 112.85571 Photosensor reading: 3096.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-90.032) Image 13. Time: Wed Nov 10 17:09:30 2021 Time Point: 8.375 secs Intensity: Min = 90, Max = 255, Mean = 112.97893 Photosensor reading: 2979.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-90.329) Image 14. Time: Wed Nov 10 17:09:31 2021 Time Point: 9.076 secs Intensity: Min = 90, Max = 248, Mean = 113.01482 Photosensor reading: 3372.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-90.630) Image 15. Time: Wed Nov 10 17:09:31 2021 Time Point: 9.777 secs Intensity: Min = 90, Max = 237, Mean = 113.05387 Photosensor reading: 3610.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-90.930) Image 16. Time: Wed Nov 10 17:09:32 2021 Time Point: 10.478 secs Intensity: Min = 91, Max = 231, Mean = 113.18955 Photosensor reading: 3326.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-91.230) Image 17. Time: Wed Nov 10 17:09:33 2021 Time Point: 11.178 secs Intensity: Min = 91, Max = 223, Mean = 113.22021 Photosensor reading: 3002.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-91.530) Image 18. Time: Wed Nov 10 17:09:34 2021 Time Point: 11.911 secs Intensity: Min = 91, Max = 220, Mean = 113.38141 Photosensor reading: 3160.0 Exposure time: 0.200000 secs EX filter: DAPI EM filter: DAPI ND filter: 10% Stage coordinates: (-11171.930,-2997.650,-91.830) Image 19. Time: Wed Nov 10 17:09:37 2021 Time Point: 13.277 secs Intensity: Min = 98, Max = 136, Mean = 111.79728 Photosensor reading: 573925.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-86.733) Image 20. Time: Wed Nov 10 17:09:39 2021 Time Point: 15.799 secs Intensity: Min = 95, Max = 138, Mean = 111.72345 Photosensor reading: 573655.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-87.030) Image 21. Time: Wed Nov 10 17:09:42 2021 Time Point: 18.335 secs Intensity: Min = 98, Max = 135, Mean = 111.72457 Photosensor reading: 573700.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-87.330) Image 22. Time: Wed Nov 10 17:09:44 2021 Time Point: 20.837 secs Intensity: Min = 96, Max = 134, Mean = 111.69172 Photosensor reading: 572879.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-87.631) Image 23. Time: Wed Nov 10 17:09:47 2021 Time Point: 23.373 secs Intensity: Min = 98, Max = 133, Mean = 111.76399 Photosensor reading: 574419.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-87.931) Image 24. Time: Wed Nov 10 17:09:49 2021 Time Point: 25.875 secs Intensity: Min = 97, Max = 133, Mean = 111.75694 Photosensor reading: 574834.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-88.231) Image 25. Time: Wed Nov 10 17:09:52 2021 Time Point: 28.410 secs Intensity: Min = 96, Max = 133, Mean = 111.74429 Photosensor reading: 573289.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-88.531) Image 26. Time: Wed Nov 10 17:09:54 2021 Time Point: 30.913 secs Intensity: Min = 96, Max = 135, Mean = 111.80624 Photosensor reading: 574197.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-88.829) Image 27. Time: Wed Nov 10 17:09:57 2021 Time Point: 33.449 secs Intensity: Min = 97, Max = 138, Mean = 111.82894 Photosensor reading: 573482.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-89.129) Image 28. Time: Wed Nov 10 17:09:59 2021 Time Point: 35.951 secs Intensity: Min = 96, Max = 136, Mean = 111.76638 Photosensor reading: 573460.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-89.432) Image 29. Time: Wed Nov 10 17:10:02 2021 Time Point: 38.486 secs Intensity: Min = 97, Max = 142, Mean = 111.83250 Photosensor reading: 573963.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-89.732) Image 30. Time: Wed Nov 10 17:10:04 2021 Time Point: 40.989 secs Intensity: Min = 95, Max = 142, Mean = 111.75327 Photosensor reading: 573061.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-90.032) Image 31. Time: Wed Nov 10 17:10:07 2021 Time Point: 43.543 secs Intensity: Min = 97, Max = 142, Mean = 111.83327 Photosensor reading: 573899.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-90.329) Image 32. Time: Wed Nov 10 17:10:10 2021 Time Point: 46.061 secs Intensity: Min = 98, Max = 142, Mean = 111.81402 Photosensor reading: 573954.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-90.630) Image 33. Time: Wed Nov 10 17:10:12 2021 Time Point: 48.597 secs Intensity: Min = 96, Max = 146, Mean = 111.83361 Photosensor reading: 574706.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-90.930) Image 34. Time: Wed Nov 10 17:10:15 2021 Time Point: 51.099 secs Intensity: Min = 97, Max = 149, Mean = 111.88409 Photosensor reading: 575036.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-91.230) Image 35. Time: Wed Nov 10 17:10:17 2021 Time Point: 53.635 secs Intensity: Min = 98, Max = 140, Mean = 111.91457 Photosensor reading: 575033.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-91.530) Image 36. Time: Wed Nov 10 17:10:20 2021 Time Point: 56.137 secs Intensity: Min = 98, Max = 140, Mean = 111.98260 Photosensor reading: 574626.0 Exposure time: 2.000000 secs EX filter: TRITC EM filter: TRITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-91.830) Image 37. Time: Wed Nov 10 17:10:21 2021 Time Point: 59.239 secs Intensity: Min = 91, Max = 120, Mean = 105.35275 Photosensor reading: 629112.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-86.733) Image 38. Time: Wed Nov 10 17:10:22 2021 Time Point: 60.040 secs Intensity: Min = 93, Max = 119, Mean = 105.21312 Photosensor reading: 626712.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-87.030) Image 39. Time: Wed Nov 10 17:10:23 2021 Time Point: 60.858 secs Intensity: Min = 93, Max = 122, Mean = 105.15768 Photosensor reading: 626210.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-87.330) Image 40. Time: Wed Nov 10 17:10:23 2021 Time Point: 61.658 secs Intensity: Min = 93, Max = 119, Mean = 105.13864 Photosensor reading: 630522.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-87.631) Image 41. Time: Wed Nov 10 17:10:24 2021 Time Point: 62.459 secs Intensity: Min = 93, Max = 120, Mean = 105.12022 Photosensor reading: 627791.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-87.931) Image 42. Time: Wed Nov 10 17:10:25 2021 Time Point: 63.277 secs Intensity: Min = 93, Max = 119, Mean = 105.05772 Photosensor reading: 629449.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-88.231) Image 43. Time: Wed Nov 10 17:10:26 2021 Time Point: 64.110 secs Intensity: Min = 92, Max = 118, Mean = 105.06830 Photosensor reading: 630189.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-88.531) Image 44. Time: Wed Nov 10 17:10:27 2021 Time Point: 64.911 secs Intensity: Min = 92, Max = 119, Mean = 105.02123 Photosensor reading: 623710.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-88.829) Image 45. Time: Wed Nov 10 17:10:27 2021 Time Point: 65.730 secs Intensity: Min = 92, Max = 120, Mean = 105.05733 Photosensor reading: 627917.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-89.129) Image 46. Time: Wed Nov 10 17:10:28 2021 Time Point: 66.565 secs Intensity: Min = 93, Max = 119, Mean = 105.03647 Photosensor reading: 627888.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-89.432) Image 47. Time: Wed Nov 10 17:10:29 2021 Time Point: 67.381 secs Intensity: Min = 92, Max = 120, Mean = 105.06176 Photosensor reading: 629160.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-89.732) Image 48. Time: Wed Nov 10 17:10:30 2021 Time Point: 68.198 secs Intensity: Min = 91, Max = 120, Mean = 105.04801 Photosensor reading: 628044.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-90.032) Image 49. Time: Wed Nov 10 17:10:31 2021 Time Point: 69.016 secs Intensity: Min = 93, Max = 120, Mean = 105.09177 Photosensor reading: 627248.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-90.329) Image 50. Time: Wed Nov 10 17:10:32 2021 Time Point: 69.833 secs Intensity: Min = 92, Max = 120, Mean = 105.09799 Photosensor reading: 630128.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-90.630) Image 51. Time: Wed Nov 10 17:10:32 2021 Time Point: 70.651 secs Intensity: Min = 93, Max = 121, Mean = 105.11209 Photosensor reading: 629915.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-90.930) Image 52. Time: Wed Nov 10 17:10:33 2021 Time Point: 71.451 secs Intensity: Min = 93, Max = 122, Mean = 105.10532 Photosensor reading: 631638.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-91.230) Image 53. Time: Wed Nov 10 17:10:34 2021 Time Point: 72.269 secs Intensity: Min = 93, Max = 122, Mean = 105.10428 Photosensor reading: 626809.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-91.530) Image 54. Time: Wed Nov 10 17:10:35 2021 Time Point: 73.069 secs Intensity: Min = 93, Max = 121, Mean = 105.12392 Photosensor reading: 628419.0 Exposure time: 0.300000 secs EX filter: FITC EM filter: FITC ND filter: 100% Stage coordinates: (-11171.930,-2997.650,-91.830) Total number of images saved: 54