Multiprocessor-enabled deconvolution (using 8 processors) decon3d: 3d deconvolution... Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 ChI Sel? Proc? WPass WavI WavO 0 0 1 0 457 457 1 1 1 0 617 617 2 0 0 0 0 0 3 0 0 0 0 0 4 0 0 0 0 0 5 0 0 0 0 0 6 0 0 0 0 0 7 0 0 0 0 0 8 0 0 0 0 0 9 0 0 0 0 0 10 0 0 0 0 0 11 0 0 0 0 0 Passed camera intensity offset 100.000000 is greater than min intensity 91.000000... Resetting all channels to default offset. Image Correction Input image file............................. /data1/Stojic_lab/Claudia_Kutter/09Nov2021_cells/A375_linc326_001_1_R3D.dv Output image file............................ /tmp/A375_linc326_001_1_R3D_D3D.dv_cor_RGL Polynomial order............................. 5 X min and max for normalization.............. 0 895 Y min and max for normalization.............. 0 895 Normalization................................ on Type of normalization........................ 1 Camera Intensity Offset (bias)............... 0.00 Bleach correction............................ on Z-line correction............................ on Intensity offset(s).......................... 0.00 50.00 Photosensor reference value(s)............... 2.8490e+03 5.6331e+05 Correcting time point 1, wavelength 1, panel 1... Reading 3D image data (37 sections)... Photobleaching correction disabled. Best-fit slope is <0 (-8.13585e-06). Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 132.22 132.22 2849.0 2849.0 1.0000/1.0000 2 132.48 132.22 3245.0 3238.5 0.8780/0.9980 3 132.52 132.21 3529.0 3520.9 0.8073/0.9977 4 132.86 132.21 3198.0 3182.3 0.8909/0.9951 5 132.93 132.21 2878.0 2862.5 0.9899/0.9946 6 132.95 132.21 3039.0 3022.0 0.9375/0.9944 7 133.58 132.21 3412.0 3377.1 0.8350/0.9898 8 133.75 132.21 3330.0 3291.7 0.8556/0.9885 9 133.98 132.21 2929.0 2890.4 0.9727/0.9868 10 134.32 132.21 2881.0 2835.7 0.9889/0.9843 11 134.42 132.21 3302.0 3247.7 0.8628/0.9835 12 135.00 132.21 3525.0 3452.0 0.8082/0.9793 13 134.86 132.21 3160.0 3097.8 0.9016/0.9803 14 135.59 132.20 2902.0 2829.6 0.9817/0.9751 15 135.40 132.20 3080.0 3007.4 0.9250/0.9764 16 135.99 132.20 3428.0 3332.5 0.8311/0.9721 17 135.80 132.20 3304.0 3216.5 0.8623/0.9735 18 136.41 132.20 2867.0 2778.5 0.9937/0.9691 19 136.51 132.20 2862.0 2771.7 0.9955/0.9684 20 136.69 132.20 3278.0 3170.3 0.8691/0.9671 21 137.08 132.20 3456.0 3333.0 0.8244/0.9644 22 137.31 132.20 3145.0 3027.9 0.9059/0.9628 23 137.54 132.19 2853.0 2742.2 0.9986/0.9612 24 137.92 132.19 3124.0 2994.2 0.9120/0.9585 25 138.28 132.19 3454.0 3301.9 0.8248/0.9560 26 138.44 132.19 3297.0 3148.1 0.8641/0.9548 27 138.69 132.19 2855.0 2721.1 0.9979/0.9531 28 138.92 132.19 2851.0 2712.8 0.9993/0.9515 29 139.54 132.19 3324.0 3148.9 0.8571/0.9473 30 139.52 132.19 3440.0 3259.1 0.8282/0.9474 31 139.87 132.19 3087.0 2917.3 0.9229/0.9450 32 139.88 132.18 2870.0 2712.1 0.9927/0.9450 33 140.46 132.18 3164.0 2977.5 0.9004/0.9411 34 140.77 132.18 3478.0 3265.8 0.8191/0.9390 35 140.85 132.18 3276.0 3074.3 0.8697/0.9384 36 141.08 132.18 2882.0 2700.2 0.9885/0.9369 37 141.29 132.18 2903.0 2715.7 0.9814/0.9355 Correcting time point 1, wavelength 2, panel 1... Reading 3D image data (37 sections)... Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 119.82 119.82 563308.0 563308.0 1.0000/1.0000 2 119.64 119.82 564027.0 564871.0 0.9987/1.0015 3 119.53 119.82 563819.0 565145.9 0.9991/1.0024 4 119.45 119.82 563945.0 565682.5 0.9989/1.0031 5 119.40 119.82 564185.0 566156.4 0.9984/1.0035 6 119.29 119.82 564312.0 566777.9 0.9982/1.0044 7 119.26 119.82 564539.0 567175.4 0.9978/1.0047 8 119.17 119.82 563935.0 566978.2 0.9989/1.0054 9 119.09 119.82 564005.0 567423.7 0.9988/1.0061 10 119.05 119.82 564665.0 568290.6 0.9976/1.0064 11 119.08 119.82 565155.0 568666.4 0.9967/1.0062 12 118.99 119.82 564055.0 567950.1 0.9987/1.0069 13 118.97 119.82 563834.0 567856.5 0.9991/1.0071 14 118.94 119.82 563978.0 568149.0 0.9988/1.0074 15 118.93 119.82 564376.0 568571.1 0.9981/1.0074 16 118.87 119.82 564745.0 569226.6 0.9975/1.0079 17 118.92 119.82 566172.0 570450.6 0.9949/1.0076 18 118.81 119.82 563995.0 568778.7 0.9988/1.0085 19 118.81 119.82 563626.0 568376.4 0.9994/1.0084 20 118.74 119.82 563557.0 568673.2 0.9996/1.0091 21 118.73 119.82 565801.0 570951.8 0.9956/1.0091 22 118.72 119.82 564951.0 570176.8 0.9971/1.0093 23 118.73 119.82 564141.0 569277.7 0.9985/1.0091 24 118.77 119.82 565725.0 570722.6 0.9957/1.0088 25 118.68 119.82 564222.0 569619.1 0.9984/1.0096 26 118.65 119.82 563849.0 569374.5 0.9990/1.0098 27 118.64 119.82 563969.0 569545.4 0.9988/1.0099 28 118.64 119.82 563945.0 569519.6 0.9989/1.0099 29 118.57 119.82 563685.0 569581.7 0.9993/1.0105 30 118.58 119.82 564318.0 570199.4 0.9982/1.0104 31 118.54 119.82 563518.0 569598.7 0.9996/1.0108 32 118.49 119.82 563515.0 569811.4 0.9996/1.0112 33 118.51 119.82 564615.0 570832.5 0.9977/1.0110 34 118.49 119.82 563355.0 569645.1 0.9999/1.0112 35 118.51 119.82 564917.0 571141.1 0.9972/1.0110 36 118.49 119.82 563756.0 570082.0 0.9992/1.0112 37 118.44 119.82 563230.0 569768.1 1.0001/1.0116 ---------------------------------------------------- Deconvolution Results: Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 Transferring field 3: COR_BlchFitSlp f 8 0.000001 Transferring field 4: COR_BlchFitInt f 8 4.775251 Transferring field 5: COR_BlchFitChi f 8 0.000000 Opening OTF file (/usr/local/otf/Olympus_100X_140_10002.otf)... decon3d: 3D iterative constrained deconvolution Input image file............................. /tmp/A375_linc326_001_1_R3D_D3D.dv_cor_RGL Output image file............................ /data1/Stojic_lab/Claudia_Kutter/09Nov2021_cells/A375_linc326_001_1_R3D_D3D.dv Method (1=add,2=ratio,3=enh add,4=enh ratio). 4 Values subtracted from image................. 0.00 0.00 Intensity scaling factor..................... 1.0000 Border roll-off size (pixels)................ 28 Pre-filter cut-off values (microns).......... 0.2000, 0.0500 Sigma for smoothing.......................... 0.3050 Sigma for smoothing Wiener filter............ 0.1100 S:N value for Wiener filter.................. 0.1010 Z padding.................................... 128 Number of iterations before smoothing........ 5 Maximum number of iterations................. 10 Autoscaling.................................. enabled Allocating image memory (nx=896,ny=896,sOpt->nzo=37,total=28.3 MP)... Generating pre-filter and smoothing function... Wavelength 457.0 nm processed with OTF measured at 617.0 nm (scale=1.350)... Preparing OTF (128 128 129)... Deconvolving wavelength 457.0, time point 1 (3D)... Z noise filter threshold=1096.00 Prefiltering wavelength 457.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01855114 2.44 1.000 2 0.01075007 1.42 0.579 3 0.01045764 1.38 0.564 4 0.01032242 1.36 0.556 5 0.01021353 1.34 0.551 6 0.01029283 1.35 0.555 7 0.01017475 1.34 0.548 8 0.01007977 1.33 0.543 9 0.00999542 1.32 0.539 10 0.00991997 1.31 0.535 decon3d: finished iterations (10/10). Wavelength 617.0 nm processed with OTF measured at 617.0 nm (scale=1.000)... Preparing OTF (128 128 129)... Deconvolving wavelength 617.0, time point 1 (3D)... Z noise filter threshold=1112.00 Prefiltering wavelength 617.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.01132377 1.35 1.000 2 0.01091674 1.30 0.964 3 0.01077839 1.28 0.952 4 0.01068711 1.27 0.944 5 0.01064722 1.27 0.940 6 0.01068381 1.27 0.943 7 0.01063371 1.26 0.939 8 0.01059848 1.26 0.936 9 0.01056824 1.26 0.933 10 0.01054261 1.25 0.931 decon3d: finished iterations (10/10). Total deconvolution time: 00:00:12