Multiprocessor-enabled deconvolution (using 8 processors) decon3d: 3d deconvolution... Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 Transferring field 3: COR_BlchFitSlp f 8 0.000001 Transferring field 4: COR_BlchFitInt f 8 4.775251 Transferring field 5: COR_BlchFitChi f 8 0.000000 ChI Sel? Proc? WPass WavI WavO 0 0 1 0 457 457 1 1 1 0 617 617 2 0 0 0 0 0 3 0 0 0 0 0 4 0 0 0 0 0 5 0 0 0 0 0 6 0 0 0 0 -0 7 0 0 0 0 0 8 0 0 0 -0 -0 9 0 0 0 0 0 10 0 0 0 0 0 11 0 0 0 0 0 Passed camera intensity offset 100.000000 is greater than min intensity 57.704838... Resetting all channels to default offset. Image Correction Input image file............................. /data1/Stojic_lab/Claudia_Kutter/09Nov2021_cells/A375_linc326_001_1_R3D_D3D.dv Output image file............................ /tmp/A375_linc326_001_1_R3D_D3D.dv_cor_ESA Polynomial order............................. 5 X min and max for normalization.............. 0 895 Y min and max for normalization.............. 0 895 Normalization................................ on Type of normalization........................ 1 Camera Intensity Offset (bias)............... 0.00 Bleach correction............................ on Z-line correction............................ on Intensity offset(s).......................... 0.00 50.00 Photosensor reference value(s)............... 2.8490e+03 5.6331e+05 Correcting time point 1, wavelength 1, panel 1... Reading 3D image data (37 sections)... Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 124.61 124.61 2849.0 2849.0 1.0000/1.0000 2 124.22 124.61 3245.0 3255.2 0.8780/1.0031 3 124.32 124.61 3529.0 3537.4 0.8073/1.0024 4 124.47 124.61 3198.0 3201.6 0.8909/1.0011 5 124.44 124.61 2878.0 2882.1 0.9899/1.0014 6 124.40 124.61 3039.0 3044.1 0.9375/1.0017 7 124.51 124.61 3412.0 3414.7 0.8350/1.0008 8 124.52 124.61 3330.0 3332.6 0.8556/1.0008 9 124.53 124.61 2929.0 2930.9 0.9727/1.0007 10 124.56 124.61 2881.0 2882.2 0.9889/1.0004 11 124.52 124.61 3302.0 3304.5 0.8628/1.0008 12 124.56 124.61 3525.0 3526.5 0.8082/1.0004 13 124.57 124.61 3160.0 3161.1 0.9016/1.0004 14 124.65 124.61 2902.0 2901.2 0.9817/0.9997 15 124.62 124.61 3080.0 3079.8 0.9250/0.9999 16 124.64 124.61 3428.0 3427.2 0.8311/0.9998 17 124.63 124.61 3304.0 3303.6 0.8623/0.9999 18 124.69 124.61 2867.0 2865.2 0.9937/0.9994 19 124.69 124.61 2862.0 2860.3 0.9955/0.9994 20 124.69 124.61 3278.0 3275.8 0.8691/0.9993 21 124.76 124.61 3456.0 3452.0 0.8244/0.9988 22 124.76 124.61 3145.0 3141.3 0.9059/0.9988 23 124.77 124.61 2853.0 2849.4 0.9986/0.9987 24 124.81 124.61 3124.0 3119.1 0.9120/0.9984 25 124.82 124.61 3454.0 3448.1 0.8248/0.9983 26 124.82 124.61 3297.0 3291.6 0.8641/0.9983 27 124.81 124.61 2855.0 2850.4 0.9979/0.9984 28 124.87 124.61 2851.0 2845.0 0.9993/0.9979 29 124.96 124.61 3324.0 3314.8 0.8571/0.9972 30 124.92 124.61 3440.0 3431.6 0.8282/0.9976 31 124.91 124.61 3087.0 3079.6 0.9229/0.9976 32 124.86 124.61 2870.0 2864.4 0.9927/0.9980 33 124.90 124.61 3164.0 3156.6 0.9004/0.9977 34 124.90 124.61 3478.0 3470.1 0.8191/0.9977 35 124.75 124.61 3276.0 3272.3 0.8697/0.9989 36 124.80 124.61 2882.0 2877.6 0.9885/0.9985 37 125.21 124.61 2903.0 2889.2 0.9814/0.9952 imgcorr: replaced 13 bad pixels. Replacing 13 pixels... Correcting time point 1, wavelength 2, panel 1... Reading 3D image data (37 sections)... Photobleaching correction disabled. Best-fit slope is <0 (-9.11168e-08). Z Ref Scaled Ref Photosensor Scaled Photosensor - --- ---------- ----------- ------------------ 1 112.07 112.07 563308.0 563307.9 1.0000/1.0000 2 111.54 112.07 564027.0 566680.6 0.9987/1.0047 3 111.37 112.07 563819.0 567328.0 0.9991/1.0062 4 111.43 112.07 563945.0 567165.6 0.9989/1.0057 5 111.49 112.07 564185.0 567121.3 0.9984/1.0052 6 111.52 112.07 564312.0 567081.4 0.9982/1.0049 7 111.51 112.07 564539.0 567360.7 0.9978/1.0050 8 111.45 112.07 563935.0 567061.2 0.9989/1.0055 9 111.39 112.07 564005.0 567428.9 0.9988/1.0061 10 111.39 112.07 564665.0 568112.5 0.9976/1.0061 11 111.38 112.07 565155.0 568659.6 0.9967/1.0062 12 111.34 112.07 564055.0 567727.4 0.9987/1.0065 13 111.30 112.07 563834.0 567725.7 0.9991/1.0069 14 111.31 112.07 563978.0 567794.8 0.9988/1.0068 15 111.38 112.07 564376.0 567887.4 0.9981/1.0062 16 111.46 112.07 564745.0 567847.8 0.9975/1.0055 17 111.43 112.07 566172.0 569409.7 0.9949/1.0057 18 111.35 112.07 563995.0 567627.4 0.9988/1.0064 19 111.20 112.07 563626.0 568049.1 0.9994/1.0078 20 111.11 112.07 563557.0 568427.6 0.9996/1.0086 21 111.08 112.07 565801.0 570837.7 0.9956/1.0089 22 111.15 112.07 564951.0 569604.5 0.9971/1.0082 23 111.31 112.07 564141.0 567983.1 0.9985/1.0068 24 111.40 112.07 565725.0 569116.0 0.9957/1.0060 25 111.34 112.07 564222.0 567889.1 0.9984/1.0065 26 111.21 112.07 563849.0 568188.2 0.9990/1.0077 27 111.16 112.07 563969.0 568595.2 0.9988/1.0082 28 111.17 112.07 563945.0 568524.6 0.9989/1.0081 29 111.17 112.07 563685.0 568216.8 0.9993/1.0080 30 111.14 112.07 564318.0 569023.9 0.9982/1.0083 31 111.09 112.07 563518.0 568453.2 0.9996/1.0088 32 111.06 112.07 563515.0 568619.2 0.9996/1.0091 33 111.05 112.07 564615.0 569791.1 0.9977/1.0092 34 111.05 112.07 563355.0 568502.1 0.9999/1.0091 35 110.99 112.07 564917.0 570391.1 0.9972/1.0097 36 111.00 112.07 563756.0 569179.8 0.9992/1.0096 37 111.33 112.07 563230.0 566983.2 1.0001/1.0067 imgcorr: replaced 12 bad pixels. Replacing 12 pixels... ---------------------------------------------------- Deconvolution Results: Transferring field 0: CameraType c 30 CoolSNAP_HQ2 / HQ2-ICX285 Transferring field 1: CameraGain f 8 1.000000 Transferring field 2: CameraBinning i 1 1 Transferring field 3: COR_BlchFitSlp f 8 0.000025 Transferring field 4: COR_BlchFitInt f 8 4.723734 Transferring field 5: COR_BlchFitChi f 8 0.000000 Opening OTF file (/usr/local/otf/Olympus_100X_140_10002.otf)... decon3d: 3D iterative constrained deconvolution Input image file............................. /tmp/A375_linc326_001_1_R3D_D3D.dv_cor_ESA Output image file............................ /data1/Stojic_lab/Claudia_Kutter/09Nov2021_cells/A375_linc326_001_1_R3D_D3D.dv Method (1=add,2=ratio,3=enh add,4=enh ratio). 4 Values subtracted from image................. 0.00 0.00 Intensity scaling factor..................... 1.0000 Border roll-off size (pixels)................ 26 Pre-filter cut-off values (microns).......... 0.2000, 0.0500 Sigma for smoothing.......................... 0.3050 Sigma for smoothing Wiener filter............ 0.1100 S:N value for Wiener filter.................. 0.1010 Z padding.................................... 128 Number of iterations before smoothing........ 5 Maximum number of iterations................. 10 Autoscaling.................................. enabled Allocating image memory (nx=896,ny=896,sOpt->nzo=37,total=28.3 MP)... Generating pre-filter and smoothing function... Wavelength 457.0 nm processed with OTF measured at 617.0 nm (scale=1.350)... Preparing OTF (128 128 129)... Deconvolving wavelength 457.0, time point 1 (3D)... Z noise filter threshold=1096.00 Prefiltering wavelength 457.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.04535870 5.85 1.000 2 0.03664974 4.73 0.808 3 0.03443231 4.44 0.759 4 0.03301202 4.26 0.728 5 0.03281153 4.23 0.723 6 0.03351983 4.32 0.739 7 0.03262102 4.21 0.719 8 0.03214012 4.14 0.709 9 0.03173951 4.09 0.700 10 0.03140735 4.05 0.692 decon3d: finished iterations (10/10). Wavelength 617.0 nm processed with OTF measured at 617.0 nm (scale=1.000)... Preparing OTF (128 128 129)... Deconvolving wavelength 617.0, time point 1 (3D)... Z noise filter threshold=1112.00 Prefiltering wavelength 617.0... decon3d: starting iterations... Standard Avg Counts Iteration Residual (R) Residual Normalized R --------- ------------ -------- ------------ 1 0.04849747 5.66 1.000 2 0.04445479 5.19 0.917 3 0.04227485 4.93 0.872 4 0.04080799 4.76 0.841 5 0.04247626 4.96 0.876 6 0.04312539 5.03 0.889 7 0.04238302 4.95 0.874 8 0.04252906 4.96 0.877 9 0.04274726 4.99 0.881 decon3d: finished iterations (9/10). Total deconvolution time: 00:00:11