Figure S1: 
M. catarrhalis was grown to the mid-exponential phase (~5 x 108 cells/ml) in BHI medium in the presence of defensin 1 (12.5 µM) or sarcotoxin 1C (0.39 µM) before dilution in PBS containing 0.15% gelatin, and directly plated on sheep blood agar. They were cultivated for a further 15 h at 37 °C and 5% CO2 in serial dilution before manual counting of the colonies on each plate.