Data and analysis script for:

Jutfelt F, Roche DG, Clark TD, Norin T, Binning SA, Speers-Roesch B, Amcoff M, Morgan R, Andreassen AH, Sundin J (in prep) Brain cooling marginally increases acute thermal tolerance in Atlantic cod

Please refer to the manuscript for data collection methods and statistical analyses. For questions or to notify the authors if any errors are identified in the data, please contact Fredrik Jutfelt (fredrik.jutfelt@ntnu.no) and/or Dominique Roche (dom.g.roche@gmail.com).

###Thermal ramping data.csv ###

Thermal ramping profiles of Atlantic cod subjected to an acute upper thermal tolerance challenge. Note that data are missing for cods 24-27 and the beginning of the trials for cods 21-23 (the thermocouple thermometer was erroneously turned off at the start of these trials). 

columnHeading		description
-------------		-----------

time			Time in seconds
cod1			Water temperature (degree C) for cod 1
...			...
cod39			Water temperature (degree C) for cod 39

Data used to create Fig. S2.




### Brain_cooling_data.csv ###

Experimental data

columnHeading		description
-------------		-----------

date			Date at which the fish was tested (month_day_year).
fishID			Fish identity.
treatment		Treatment group: control = uninstrumented fish; ambient = instrumented control (fish were mounted with brain coolers receiving ambient, ramping-temperature water); cooled = treatment fish (fish were mounted with brain coolers receiving cooled water) 
TL			Total length (cm)
mass			Mass (g)
CTmax			Critical thermal maximum (degree C) - referred to as "acute upper thermal tolerance" in the manuscript
comment			Comments

Data used to create Table 1.




### Brain_cooling_data_for_fig.csv ###

Same as "Brain_cooling_data.csv" but each treatment is duplicated. See "bc_estimation_stats.R" for an explanation.

Data used to create Fig. 2 and Fig. S3.




### bc_estimation_stats.R ###

Annotated R script to compute estimation statistics and produce Table 1, Fig. 2, Fig. S2 and Fig. S3. 

See:  
Ho, J., Tumkaya, T., Aryal, S., Choi, H., & Claridge-Chang, A. (2019). Moving beyond P values: data analysis with estimation graphics. Nat. Methods, 16, 565-566.